Oszter A, Törocsik B, Vértes Z, Környei J L, Kovács K A, Vértes M
Institute of Physiology, University Medical School of Pécs, Pécs, Hungary.
Eur J Pharmacol. 2000 Apr 28;395(2):103-6. doi: 10.1016/s0014-2999(00)00214-4.
The present studies demonstrate, for the first time, that the binding of activator protein-1 (AP-1)-DNA in rat uterus and the estrogen-sensitive areas of the hypothalamus, as measured by electrophoretic mobility shift assay, is increased 2 h after intraperitoneal injection of [D-Met(2),Pro(5)]enkephalinamide. The effect was prevented by the opiate antagonist naltrexone given 30 min before the administration of [D-Met(2),Pro(5)]enkephalinamide, suggesting the involvement of opioid peptide receptors in the observed effects. The present findings support the role of opioid peptides in the regulation of transcription in estrogen-sensitive cells.
目前的研究首次证明，通过电泳迁移率变动分析测定，腹腔注射[D-蛋氨酸(2)，脯氨酸(5)]脑啡肽酰胺2小时后，大鼠子宫和下丘脑雌激素敏感区域中激活蛋白-1（AP-1）与DNA的结合增加。在给予[D-蛋氨酸(2)，脯氨酸(5)]脑啡肽酰胺前30分钟给予阿片拮抗剂纳曲酮可阻止该效应，提示阿片肽受体参与了观察到的效应。目前的研究结果支持阿片肽在雌激素敏感细胞转录调控中的作用。
