Vértes Z, Környei J L, Kovács S, Vértes M
Institute of Physiology, University Medical School, Pécs, Hungary.
Eur J Pharmacol. 1995 Oct 15;291(2):115-20. doi: 10.1016/0922-4106(95)90132-9.
The effects of a single dose of naloxone and of [D-Met2,Pro5]enkephalinamide on the DNA synthesis in the uterus of 7, 14 and 21-day-old rat were studied. After [D-Met2,Pro5]enkephalinamide treatment, an age-dependent decrease in in vitro [3H]thymidine incorporation into DNA was observed in all studied age groups. In the 21-day-old age group a reduced rate of DNA synthesis was detected for 12 h after [D-Met2,Pro5]enkephalinamide treatment followed by the return to control values at 24 h. The rate of inhibition was more marked in the younger age groups. The effect was also more pronounced in younger animals. Specific [3H]naloxone binding was detected both in membrane and nuclear fractions of uterine homogenates. While no age-related changes in binding affinities were found, the number of binding sites varied characteristically during development. Our data suggest the novel involvement of opioid peptides and their receptors in the regulation of uterine development.
研究了单剂量纳洛酮和[D-蛋氨酸²，脯氨酸⁵]脑啡肽酰胺对7日龄、14日龄和21日龄大鼠子宫DNA合成的影响。经[D-蛋氨酸²，脯氨酸⁵]脑啡肽酰胺处理后，在所有研究的年龄组中均观察到体外[³H]胸腺嘧啶核苷掺入DNA的量随年龄增长而减少。在21日龄组中，[D-蛋氨酸²，脯氨酸⁵]脑啡肽酰胺处理后12小时检测到DNA合成速率降低，随后在24小时恢复到对照值。抑制率在较年轻的年龄组中更为明显。这种效应在较年轻的动物中也更为显著。在子宫匀浆的膜和核组分中均检测到特异性[³H]纳洛酮结合。虽然未发现结合亲和力存在与年龄相关的变化，但结合位点的数量在发育过程中具有特征性变化。我们的数据表明阿片肽及其受体在子宫发育调节中具有新的作用。
