高效选择CD4和CD8谱系胸腺细胞支持谱系定向的指导模型。

Highly efficient selection of CD4 and CD8 lineage thymocytes supports an instructive model of lineage commitment.

作者信息

Itano A, Robey E

机构信息

Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.

出版信息

Immunity. 2000 Apr;12(4):383-9. doi: 10.1016/s1074-7613(00)80190-9.

DOI:10.1016/s1074-7613(00)80190-9

PMID:10795736

Abstract

We undertook a kinetic analysis of the generation of mature T cells in TCR and coreceptor transgenic mice using BrdU labeling. We observed that the selection efficiency of mature CD4-CD8+ and CD4+CD8- thymocytes could be as high as 40% and 90% of CD4+CD8+ precursors, respectively. The surprisingly high efficiency of selection favors an instructional model of lineage commitment and is incompatible with a stochastic model in which the efficiency of selection would be no greater than 100% in both lineages combined.

摘要

我们使用BrdU标记对TCR和共受体转基因小鼠中成熟T细胞的生成进行了动力学分析。我们观察到，成熟的CD4-CD8+和CD4+CD8-胸腺细胞的选择效率分别可高达CD4+CD8+前体细胞的40%和90%。这种惊人的高选择效率支持谱系定向的指导模型，并且与随机模型不相符，在随机模型中，两个谱系的选择效率加起来不会超过100%。