Lane J L
Br J Haematol. 1978 Nov;40(3):459-70. doi: 10.1111/j.1365-2141.1978.tb05817.x.
Immunoelectrophoretic studies were carried out on plasma from a patient with an abnormal antithrombin III molecule (antithrombin III 'Budapest'). One-dimensional immunoelectrophoresis using a commercial antibody to antithrombin III (antibody to alpha2-AtIII) showed two precipitin peaks of alpha2-AtIII antigen. Two-dimensional immunoelectrophoresis showed two precipitin peaks, one with normal electrophoretic mobility, the other with increased electrophoretic mobility. It was shown that alpha2-AtIII antigen with normal mobility was identical to normal alpha2-AtIII, and that the alpha2-AtIII antigen with increased electrophoretic mobility was antigenically deficient, and appeared to be present in higher concentration than the normal alpha2-AtIII antigen. Although one-dimensional immunoelectrophoresis on plasma from the patient's son showed only one peak, two-dimensional immunoelectrophoresis revealed that the son also had two populations of alpha2-AtIII, one with normal mobility, the other with increased mobility. However, the alpha2-AtIII antigen with normal mobility appeared to be present in higher concentration than the alpha2-AtIII antigen with increased mobility. One- and two-dimensional immunoelectrophoresis using a different commercial antibody to alpha2-AtIII showed only one precipitin peak using the patient's plasma. The precipitin peak observed following two-deminsional immunoelectrophoresis was asymmetric and showed increased mobility.
对一名抗凝血酶III分子异常（抗凝血酶III“布达佩斯”）患者的血浆进行了免疫电泳研究。使用抗抗凝血酶III的商业抗体（抗α2-抗凝血酶III抗体）进行的一维免疫电泳显示出α2-抗凝血酶III抗原的两个沉淀峰。二维免疫电泳显示出两个沉淀峰，一个具有正常的电泳迁移率，另一个电泳迁移率增加。结果表明，具有正常迁移率的α2-抗凝血酶III抗原与正常的α2-抗凝血酶III相同，而电泳迁移率增加的α2-抗凝血酶III抗原在抗原性上存在缺陷，并且其浓度似乎高于正常的α2-抗凝血酶III抗原。尽管对患者儿子的血浆进行的一维免疫电泳仅显示一个峰，但二维免疫电泳显示该儿子也有两种α2-抗凝血酶III，一种具有正常迁移率，另一种迁移率增加。然而，具有正常迁移率的α2-抗凝血酶III抗原的浓度似乎高于迁移率增加的α2-抗凝血酶III抗原。使用另一种抗α2-抗凝血酶III的商业抗体对患者血浆进行一维和二维免疫电泳，仅显示一个沉淀峰。二维免疫电泳后观察到的沉淀峰不对称，且迁移率增加。
