[大鼠碳酸酐酶I和II的放射免疫测定。在个体发育过程中对中枢神经系统的应用]

[Radioimmunoassay of carbonic anhydrase I and II in rats. Application to the central nervous system during ontogeny].

作者信息

Limozin N, Filippi D, Dalmasso C, Laurent G

出版信息

Biochimie. 1979;61(1):115-9. doi: 10.1016/s0300-9084(79)80320-x.

Abstract

A specific radioimmunoassay method for rat erythrocyte carbonic anhydrases I and II was developed using a double antibody system. Its sensitivity was in the nanogram range for each of the two isozymes. The method has been applied to the assay of cerebral carbonic anhydrase. Only CAII has been found in brain extracts of perfused rats. Accordingly, the assay of CAI in cerebral tissue can be used to quantify erythrocyte contamination on condition that the ratio CAII/CAI in blood had been worked out. The developmental change in the soluble and the Triton X-100 solubilized brain CAII from birth to adult is reported.

摘要

利用双抗体系统开发了一种用于检测大鼠红细胞碳酸酐酶I和II的特异性放射免疫分析方法。该方法对两种同工酶的检测灵敏度均在纳克范围内。此方法已应用于脑碳酸酐酶的检测。在灌流大鼠的脑提取物中仅发现了CAII。因此，在确定血液中CAII/CAI的比例后，脑组织中CAI的检测可用于量化红细胞污染情况。本文报道了从出生到成年期可溶性和经Triton X-100增溶的脑CAII的发育变化。