Langley O K, Ghandour M S, Vincendon G, Gombos G
Histochem J. 1980 Jul;12(4):473-83. doi: 10.1007/BF01011962.
The cellular and intracellular distribution of carbonic anhydrase isozyme II in rat cerebellum has been investigated with the electron microscope by the indirect antibody immunohistochemical technique. Unequivocal evidence is presented supporting the view that this enzyme is exclusively localized in oligodendrocytes. Myelin does not appear to contain detectable amounts of carbonic anhydrase though it is present in oligodendrocyte processes and in the layer of oligodendrocyte cytoplasm frequently seen to coat the external surface of myelinated fibres. The immune precipitate is found to be confined to the cytosol and the cytosolic surfaces of intracellular membranes. The data are discussed in relation to the possible function of the enzyme and the role of oligodendrocytes in the central nervous system.
采用间接抗体免疫组织化学技术，通过电子显微镜研究了大鼠小脑中碳酸酐酶同工酶II的细胞和细胞内分布。提供了明确的证据支持这种酶仅定位于少突胶质细胞的观点。髓磷脂似乎不含有可检测量的碳酸酐酶，尽管它存在于少突胶质细胞突起以及经常可见覆盖有髓鞘纤维外表面的少突胶质细胞质层中。发现免疫沉淀物局限于细胞质溶胶和细胞内膜的细胞质溶胶表面。结合该酶的可能功能以及少突胶质细胞在中枢神经系统中的作用对这些数据进行了讨论。
