Mennecier F, Weber A, Tudury C, Dreyfus J C
Biochimie. 1979;61(1):79-85. doi: 10.1016/s0300-9084(79)80315-6.
Crude hemolysates, partially purified aldolase and aldolase purified to homogeneity from reticulocytes and mature erythrocytes, were incubated with a specific antiserum raised against crystalline rabbit muscle aldolase. We show that the same aldolasic activity corresponds to a greater amount of antigen in older than in younger cells, in crude hemolysates as well as in the above mentioned preparations; that is to say, old-cell aldolase contains cross-reacting material (CRM). Properties of purified enzyme from reticulocytes and mature erythrocytes were compared to those of muscle crystalline aldolase: -- the molecular specific activity of purified aldolase from erythrocytes is lower than with crystalline muscle aldolase, i.e. CRM is maintained throughout the purification steps. -- the specific activity of red cell aldolase towards both substrates (FDP and F1P) is lower than that of crystalline muscle aldolase. However, the ratio of activity towards the two substrates FDP/F1P is decreased in erythrocytes and reticulocytes. -- no other difference was found: Michaelis constant towards FDP, thermodenaturation constant and C terminal extremities are identical as are the molecular weights.
将来自网织红细胞和成熟红细胞的粗溶血产物、部分纯化的醛缩酶以及纯化至同质的醛缩酶与针对结晶兔肌肉醛缩酶产生的特异性抗血清一起孵育。我们发现，在粗溶血产物以及上述制剂中，相同的醛缩酶活性在较老细胞中对应的抗原量比在较年轻细胞中更多；也就是说，老细胞醛缩酶含有交叉反应物质（CRM）。将来自网织红细胞和成熟红细胞的纯化酶的性质与肌肉结晶醛缩酶的性质进行了比较：——红细胞纯化醛缩酶的分子比活性低于结晶肌肉醛缩酶，即CRM在整个纯化步骤中都得以保留。——红细胞醛缩酶对两种底物（FDP和F1P）的比活性低于结晶肌肉醛缩酶。然而，红细胞和网织红细胞中对两种底物FDP/F1P的活性比降低。——未发现其他差异：对FDP的米氏常数、热变性常数和C末端相同，分子量也相同。
