Weber A, Gregori C, Schapira F
Biochim Biophys Acta. 1976 Oct 22;444(3):810-5. doi: 10.1016/0304-4165(76)90328-7.
The process of enzymatic aging was studied in livers of adult and senescent rats for aldolase B. No "cross-reacting material" was found in livers of 27 to 30-month-old rats, estimated by the ratio aldolase activity-antigen amount. The activity towards the two substrates of aldolase, fructose 1,6-diphosphate and fructose 1-phosphate did not vary in senescent animals. Moreover, other physico-chemical properties of the enzyme such as thermal inactivation, immunological reactivity and Michaelis constant remain unchanged. These results provide arguments against the occurrence of errors in protein synthesis as a cause of aging.
在成年和老年大鼠的肝脏中研究了醛缩酶B的酶促老化过程。通过醛缩酶活性与抗原量的比率估计，在27至30月龄大鼠的肝脏中未发现“交叉反应物质”。在老年动物中，醛缩酶对两种底物果糖1,6-二磷酸和果糖1-磷酸的活性没有变化。此外，该酶的其他物理化学性质，如热失活、免疫反应性和米氏常数保持不变。这些结果为蛋白质合成错误是衰老原因这一观点提供了反证。
