Figarella C, Negri G A, Guy O
Eur J Biochem. 1975 May 6;53(2):457-63. doi: 10.1111/j.1432-1033.1975.tb04086.x.
The two human anionic trypsinogens 1 and 2 were purified from human pancreatic juice by gel filtration on Sephadex G-100 and by chromatography on DEAE-cellulose. After activation of their respective zymogens by porcine enterokinase, human trypsins 1 and 2 were studied for their reaction with a wide variety of proteinase inhibitors. Kunitz pancreatic trypsin inhibitor and human pancreatic secretory trypsin inhibitor completely inhibited both human trypsins at a stoichiometric inhibitor-to-enzyme ratio of one to one. In contrast, bovine pancreatic secretory trypsin inhibitor (Kazal's inhibitor) failed to inhibit either human trypsin. The inhibition of both human trypsins by porcine pancreatic secretory trypsin inhibitor was demonstrated. The reactions of the trypsins with chicken ovomucoid, Ascaris lumbricoides (type suis), human sperm and blood plasma trypsin inhibitors were studied. The most striking difference between the two human trypsins was the reaction with soybean trypsin inhibitor (Kunitz). Trypsin 2 was completely inhibited in a one-to-one molar ratio while trypsin 1 was poorly inhibited. The presence of a prekallikrein in human pancreatic juice is discussed.
通过在葡聚糖凝胶G - 100上进行凝胶过滤以及在二乙氨基乙基纤维素上进行层析，从人胰液中纯化出两种人阴离子胰蛋白酶原1和2。在用猪肠激酶激活各自的酶原后，研究了人胰蛋白酶1和2与多种蛋白酶抑制剂的反应。库尼茨胰蛋白酶抑制剂和人胰分泌性胰蛋白酶抑制剂以1:1的化学计量抑制剂与酶比例完全抑制了两种人胰蛋白酶。相比之下，牛胰分泌性胰蛋白酶抑制剂（卡扎尔抑制剂）未能抑制任何一种人胰蛋白酶。已证明猪胰分泌性胰蛋白酶抑制剂对两种人胰蛋白酶均有抑制作用。研究了胰蛋白酶与鸡卵类粘蛋白、猪蛔虫（猪型）、人精子和血浆胰蛋白酶抑制剂的反应。两种人胰蛋白酶之间最显著的差异是与大豆胰蛋白酶抑制剂（库尼茨）的反应。胰蛋白酶2以1:1的摩尔比被完全抑制，而胰蛋白酶1的抑制作用较弱。文中讨论了人胰液中激肽释放酶原的存在。
