Hussain S, Ehrenberg L
Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Apr;27(4):355-62. doi: 10.1080/09553007514550321.
The prophage inducing efficiency in E. coli K-12 (lambda) of a number of agents--alkylating agents and radiations--has been compared at a high survival of bacteria. The inducing effectiveness (per alkylation in DNA) and efficiency (compared with mutation frequency in E. coli Sd-4) was found to decrease in the order 2-hydroxyethylating agents (2-hydroxyethyl methanesulphonate and ethylene oxide) greater than isopropyl methanesulphonate approximately equal to methyl methanesulphonate greater than ethylating agents (diethyl sulphate, ethyl methanesulphonate). The low inducing activity of the ethylating agents could not be explained with respect to their reactivity towards targets of differing nucleophilicity, nor could the effectiveness in mutagenicity or the ability to break the chromosomes in the presence of metal ions be invoked. The high inducing efficiency of hydroxyalkylating agents may be related to their ability to break DNA strands.
在细菌高存活率的情况下，比较了多种试剂（烷化剂和辐射）对大肠杆菌K-12（λ）的原噬菌体诱导效率。发现诱导效果（每DNA烷基化）和效率（与大肠杆菌Sd-4中的突变频率相比）按以下顺序降低：2-羟乙基化剂（甲磺酸2-羟乙酯和环氧乙烷）大于甲磺酸异丙酯，约等于甲磺酸甲酯大于乙基化剂（硫酸二乙酯、甲磺酸乙酯）。乙基化剂的低诱导活性无法根据它们对不同亲核性靶标的反应性来解释，也不能归因于其致突变性效力或在金属离子存在下断裂染色体的能力。羟烷基化剂的高诱导效率可能与其断裂DNA链的能力有关。
