120名泰国受试者HLA - DR分型的血清学和分子结果的比较分析。

Comparative analysis of serological and molecular results for HLA-DR typing in 120 Thai subjects.

作者信息

Nathalang O, Kupatawintu P, O'Charoen R, Sinsiri S, Saelee S, Tatsumi N

机构信息

Department of Pathology, Pramongkutklao College of Medicine, Bangkok, Thailand.

出版信息

Southeast Asian J Trop Med Public Health. 1999 Jun;30(2):311-3.

PMID:10774701

Abstract

In this study, serological HLA-DR typing results were compared to typing results obtained with sequence-specific primers in the polymerase chain reaction (PCR-SSP). HLA-DR typing was performed on 120 random Thai individuals. Differences in HLA-DR typing results were found in 18 out of 120, which were due to cross reactive antibodies and the lack of potent antisera to define proper HLA-DR splits by serology. Furthermore, PCR-SSP is fast and easy to perform as HLA-DR typing results can be obtained within 2 hours. From the results of this study it can be concluded that PCR-SSP is a reliable and promising technique for HLA-DR typing which can replace the serological technique in routine clinical practice.

摘要

在本研究中，将血清学HLA - DR分型结果与通过聚合酶链反应（PCR - SSP）中序列特异性引物获得的分型结果进行了比较。对120名随机选取的泰国个体进行了HLA - DR分型。120例中有18例HLA - DR分型结果存在差异，这是由于交叉反应抗体以及缺乏有效的抗血清来通过血清学确定合适的HLA - DR亚型。此外，PCR - SSP快速且易于操作，因为可在2小时内获得HLA - DR分型结果。从本研究结果可以得出结论，PCR - SSP是一种用于HLA - DR分型的可靠且有前景的技术，可在常规临床实践中取代血清学技术。