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[用于鉴定链球菌、肠球菌和葡萄球菌种类的微量方法]

[Micromethod for the identification of streptococcal, enterococcal and staphylococcal species].

作者信息

Gassama A, Boye C S, Ndao S K, Kairé O, Coly I, Macondo E A, Sow A I, Diaw L, Diop-Diop M, Mboup S

机构信息

Laboratoire de Bactériologie-Virologie, CHU Aristide Le Dantec, Daka, Sénégal.

出版信息

Dakar Med. 1999;44(1):76-83.

Abstract

The aim of this study was to set accurate and reliable methods in the identification of streptococcal, enterococcal and staphylococcal species. Micro CSB Strep and Staph system consists each of a strip with cupules containing dehydrated substrates for biochemical identification of bacterial species. Baye's theorem was used to validate tests. Reactions from micromethods were clear and easily read. Identification of 229 strains of streptococci and enterococci was correct for most species with 98.7% species with 99.3% sensitivity. 41 strains of staphylococci were also correctly identified with 85.2% of specificity and 97.68% of sensitivity.

摘要

本研究的目的是建立准确可靠的方法来鉴定链球菌、肠球菌和葡萄球菌种类。Micro CSB Strep和Staph系统各由一条带有小杯的试纸条组成,小杯中含有用于细菌种类生化鉴定的脱水底物。使用贝叶斯定理来验证测试。微量法的反应清晰且易于读取。对于大多数种类,229株链球菌和肠球菌的鉴定是正确的,物种鉴定正确率为98.7%,灵敏度为99.3%。41株葡萄球菌也被正确鉴定,特异性为85.2%,灵敏度为97.68%。

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