Hoerstrup S P, Stammberger U, Hillinger S, Zünd G, Frick R, Lachat M, Schmid R
Department of Cardiovascular Surgery, University Hospital, Zurich, Switzerland.
J Invest Surg. 2000 Mar-Apr;13(2):73-7. doi: 10.1080/089419300272113.
The development of microsurgical techniques offers a valuable opportunity to use small animals for experimental studies of vascularized organ transplants. Availability of inbred strains, natural resistance to infection, and economy make the rat an ideal animal model to investigate the effects of heart transplantation. The recent high interest and substantial laboratory activity with regard to posttransplantory immunological tissue reactions and apoptotic tissue processes led us to optimize transplantation technique by improving myocardial protection during ischemia and thereby minimizing adverse effects of the transplantation procedure itself. Thus the present report details the technique of heterotopic heart transplantation in rats using cardioplegic arrest.
显微外科技术的发展为利用小动物进行血管化器官移植的实验研究提供了宝贵机会。近交系的可获得性、对感染的天然抵抗力以及经济性，使大鼠成为研究心脏移植效果的理想动物模型。近期，人们对移植后免疫组织反应和凋亡组织过程高度关注且开展了大量实验室研究，这促使我们通过改善缺血期间的心肌保护来优化移植技术，从而将移植手术本身的不良影响降至最低。因此，本报告详细介绍了使用心脏停搏法进行大鼠异位心脏移植的技术。
