Kawakami K, Amsterdam A, Shimoda N, Becker T, Mugg J, Shima A, Hopkins N
Department of Tumor Biology, The Institute of Medical Science, The University of Tokyo, Minato-ku, 108-8639, Japan.
Curr Biol. 2000 Apr 20;10(8):463-6. doi: 10.1016/s0960-9822(00)00444-9.
The zebrafish, Danio rerio, has three types of pigment cells (melanophores, xanthophores and iridophores) and, in adult fish, these cells are organized into a stripe pattern. The mechanisms underlying formation of the stripe pattern are largely unknown. We report here the identification and characterization of a novel dominant zebrafish mutation, hagoromo (hag), which was generated by insertional mutagenesis using a pseudotyped retrovirus. The hag mutation caused disorganized stripe patterns. Two hag mutant alleles were isolated independently and proviruses were located within the fifth intron of a novel gene, which we named hag, encoding an F-box/WD40-repeat protein. The hag gene was mapped to linkage group (LG)13, close to fgf8 and pax2.1. Amino acid sequence similarity, conserved exon-intron boundaries and conserved synteny indicated that zebrafish hag is an ortholog of mouse Dactylin, the gene mutated in the Dactylaplasia (Dac) mouse [1]. The Dac mutation is dominant and causes defects in digit formation in fore- and hindlimbs. This study revealed that the hag locus is important for pattern formation in fish but is involved in distinct morphogenetic events in different vertebrates.
斑马鱼（Danio rerio）有三种色素细胞（黑素细胞、黄色素细胞和虹彩细胞），在成年鱼中，这些细胞排列成条纹图案。条纹图案形成的潜在机制在很大程度上尚不清楚。我们在此报告了一种新型显性斑马鱼突变体hagoromo（hag）的鉴定和特征，该突变体是通过使用假型逆转录病毒进行插入诱变产生的。hag突变导致条纹图案紊乱。两个hag突变等位基因被独立分离，前病毒位于一个新基因的第五个内含子内，我们将该基因命名为hag，它编码一种F-box/WD40重复蛋白。hag基因被定位到连锁群（LG）13，靠近fgf8和pax2.1。氨基酸序列相似性、保守的外显子-内含子边界和保守的同线性表明，斑马鱼hag是小鼠Dactylin的直系同源基因，Dactylin是在指发育不全（Dac）小鼠中发生突变的基因[1]。Dac突变是显性的，会导致前肢和后肢指形成缺陷。这项研究表明，hag基因座对鱼类的图案形成很重要，但在不同脊椎动物中参与不同的形态发生事件。
