Fascetti A J, Rogers Q R, Morris J G
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA 95616-8741, USA.
J Nutr. 2000 May;130(5):1287-90. doi: 10.1093/jn/130.5.1287.
The objective of this study was to determine the copper requirement of female cats (queens) for gestation. Cuproenzyme activities were evaluated to identify a noninvasive indicator of copper status. This study used a depletion-repletion model. Specific pathogen-free queens (n = 28) were adapted to a purified diet; after consuming a copper-depletion diet (0.8 mg Cu/kg diet) for 4 mo, they were randomly allocated to three dietary treatment groups receiving copper sulfate at 4.0, 5.8 or 10.8 mg Cu/kg diet. Four queens underwent liver biopsies at two time points during the study. Plasma samples were analyzed for copper concentrations, extracellular superoxide dismutase, ceruloplasmin and diamine oxidase activities. Only liver copper concentrations were responsive to dietary copper intake. The dietary concentration of copper had a significant effect on the time taken for queens to conceive (P = 0.04). There was a negative linear relationship between dietary copper (x = Cu mg/kg diet) and the mean time (y = days) for queens to conceive (y = 43.38-2.87x; R(2) = 0.97). The current NRC recommendation of 5 mg/kg diet copper for cats appears marginal for optimal reproduction.
本研究的目的是确定妊娠母猫（猫皇后）的铜需求量。评估铜酶活性以确定铜状态的非侵入性指标。本研究采用了耗竭-补充模型。无特定病原体的母猫（n = 28）适应纯化饮食；在食用4个月的低铜饮食（0.8毫克铜/千克饮食）后，将它们随机分配到三个饮食处理组，分别接受4.0、5.8或10.8毫克铜/千克饮食的硫酸铜。在研究期间的两个时间点，对4只母猫进行了肝脏活检。分析血浆样本中的铜浓度、细胞外超氧化物歧化酶、铜蓝蛋白和二胺氧化酶活性。只有肝脏铜浓度对饮食铜摄入量有反应。饮食中铜的浓度对母猫受孕所需时间有显著影响（P = 0.04）。饮食铜（x = 毫克铜/千克饮食）与母猫受孕平均时间（y = 天）之间存在负线性关系（y = 43.38 - 2.87x；R² = 0.97）。美国国家研究委员会目前建议猫的饮食铜含量为5毫克/千克，这一水平对于最佳繁殖似乎处于临界状态。
