Fascetti Andrea J, Rogers Quinton R, Morris James G
Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis 95616-8741, USA.
Vet Clin Pathol. 2002;31(4):183-8. doi: 10.1111/j.1939-165x.2002.tb00299.x.
There is little published information on plasma copper and whole blood copper concentrations in cats, and except for extracellular superoxide dismutase (EC SOD), we are unaware of published values for cat cuproenzyme activities.
In this study we determined plasma and whole blood copper concentrations, plasma ceruloplasmin (CP), EC SOD and diamine oxidase (DAO) activities, and erythrocyte superoxide dismutase (RBC SOD) activity in a specific pathogen-free colony of cats. Results were evaluated for differences based on age and sex.
Blood was obtained from 20 adult cats (10 males, 10 females; >1 year of age) and 20 immature cats (10 males, 10 females; <1 year of age). Copper concentrations were determined using atomic absorption spectrophotometry. EC SOD and RBC SOD activities were determined using the pyrogallol oxidation method, and CP activity was determined by the oxidation of 0 dianisidine dihydrochloride. DAO activity was measured using a colorimetric assay. Differences among groups were analyzed using ANOVA.
There were significant differences in mean plasma copper concentration, and CP and DAO activities (P=.001,.0001, and.02, respectively) among the 4 groups of cats. No age differences were identified. Male cats had significantly greater mean (+/- SEM) plasma copper (15.4 +/- 0.9 micromol/L versus 11.3 +/- 0.6 micromol/L; P =.001) concentrations, and CP (66.8 +/- 2.9 U/L versus 39.7 +/- 2.1 U/L; P=.0001) and DAO (6.68 +/- 0.16 U/L versus 6.15 +/- 0.1 U/L; P =.03) activities compared with female cats. Males had significantly greater whole blood copper concentrations (16.16 +/- 0.55 micromol/L versus 13.36 +/- 0.52 micromol/L; P =.002) than female cats.
Differences exist between male and female cats with respect to blood copper concentrations, and CP and DAO activities.
关于猫血浆铜和全血铜浓度的公开信息很少，除了细胞外超氧化物歧化酶（EC SOD）外，我们不知道猫铜酶活性的公开值。
在本研究中，我们测定了特定无特定病原体猫群的血浆和全血铜浓度、血浆铜蓝蛋白（CP）、EC SOD和二胺氧化酶（DAO）活性以及红细胞超氧化物歧化酶（RBC SOD）活性。根据年龄和性别对结果进行差异评估。
从20只成年猫（10只雄性，10只雌性；年龄>1岁）和20只未成年猫（10只雄性，10只雌性；年龄<1岁）采集血液。使用原子吸收分光光度法测定铜浓度。使用邻苯三酚氧化法测定EC SOD和RBC SOD活性，通过氧化0盐酸联苯胺测定CP活性。使用比色法测定DAO活性。使用方差分析分析组间差异。
四组猫的平均血浆铜浓度、CP和DAO活性存在显著差异（分别为P = 0.001、0.0001和0.02）。未发现年龄差异。雄性猫的平均（±SEM）血浆铜浓度（15.4±0.9微摩尔/升对11.3±0.6微摩尔/升；P = 0.001）、CP（66.8±2.9 U/L对39.7±2.1 U/L；P = 0.0001）和DAO（6.68±0.16 U/L对6.15±0.1 U/L；P = 0.03）活性显著高于雌性猫。雄性猫的全血铜浓度（16.16±0.55微摩尔/升对13.36±0.52微摩尔/升；P = 0.002）显著高于雌性猫。
雄性和雌性猫在血铜浓度、CP和DAO活性方面存在差异。
