McDougald D, Rice S A, Kjelleberg S
School of Microbiology and Immunology, The University of New South Wales, Sydney, Australia.
Gene. 2000 May 2;248(1-2):213-21. doi: 10.1016/s0378-1119(00)00117-7.
Vibrio vulnificus is an opportunistic pathogen that exhibits numerous virulence factors, including the secretion of a zinc metalloprotease and the production of a capsule. We have cloned and sequenced a gene from V. vulnificus that is a homologue of the positive transcriptional regulator, luxR, of the lux operon in Vibrio harveyi. This gene encodes a putative, single complete open reading frame designated smcR, which shares greater than 75% nucleotide identity with luxR of V. harveyi. The deduced amino acid sequence of the putative SmcR protein is more than 90% identical and 95% similar to that of LuxR of V. harveyi, suggesting that V. vulnificus possesses a member of the family of signal-response genes recently described in Vibrio cholerae and in Vibrio parahaemolyticus. Our data also demonstrate that, in addition to V. vulnificus, all six Vibrio spp. tested contained genes that hybridized with the luxR probe. We also present evidence that this regulatory protein was inherited from a common ancestor, and that the gene is ancient and widespread in marine Vibrio spp.
创伤弧菌是一种机会致病菌，具有多种毒力因子，包括分泌锌金属蛋白酶和产生荚膜。我们从创伤弧菌中克隆并测序了一个基因，它是哈维氏弧菌lux操纵子中正向转录调节因子luxR的同源物。该基因编码一个推定的单一完整开放阅读框，命名为smcR，它与哈维氏弧菌的luxR具有超过75%的核苷酸同一性。推定的SmcR蛋白推导的氨基酸序列与哈维氏弧菌的LuxR的氨基酸序列有超过90%的同一性和95%的相似性，这表明创伤弧菌拥有最近在霍乱弧菌和副溶血性弧菌中描述的信号应答基因家族的一个成员。我们的数据还表明，除了创伤弧菌外，所测试的所有六种弧菌属细菌都含有与luxR探针杂交的基因。我们还提供证据表明，这种调节蛋白是从一个共同祖先遗传而来的，并且该基因在海洋弧菌属中是古老且广泛存在的。
