Tsivileva O M, Nikitina V E, Garibova L V, Zav'ialova L A, Ignatov V V
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, Russia.
Mikrobiologiia. 2000 Jan-Feb;69(1):38-44.
The hemagglutinating (HA) activity of the submerged mycelium and the culture liquid (CL) of four strains of Lentinus edodes was studied. The HA activity of CLs proved to be much higher than that of mycelia. The carbohydrate specificity of fungal agglutinating factors was determined. HA activity was investigated as a function of the inoculum size, cultivation temperature, and culture age. The agglutinating activity of different morphogenetic structures of L. edodes F-249, including mycelium, brown mycelial mat (MM), primordia, and fruiting bodies, was studied, MM was found to possess the maximum HA activity, which can be explained by the possible involvement of agglutinins in the formation of MM, which is composed of glued hyphae.
研究了4株香菇深层菌丝体和培养液(CL)的血凝(HA)活性。结果表明,CL的HA活性远高于菌丝体。测定了真菌凝集因子的碳水化合物特异性。研究了HA活性与接种量、培养温度和培养时间的关系。研究了香菇F-249不同形态发生结构(包括菌丝体、褐色菌丝垫(MM)、原基和子实体)的凝集活性,发现MM具有最大的HA活性,这可能是由于凝集素参与了由胶合菌丝组成的MM的形成。