Onishchenko G G, Lomov Iu M, Markov V I, Merkulov V A, Mironov A N, Borisevich I V, Makhlaĭ A A, Vasil'ev N T, Vodop'ianov S O, Tikhonov N G, Pashanina T P, Manankov V V, Smelianskiĭ V P, Savchenko S T, Smirnova S E
Ministry of Health of the Russian Federation, Moscow.
Zh Mikrobiol Epidemiol Immunobiol. 2000 Mar-Apr(2):32-6.
The results of the molecular biological detection of the etiologic agent of hemorrhagic fever in Rostov Province are presented. The role of the causative agents of Astrakhan rickettsial fever, hemorrhagic fever with the renal syndrome, Q fever, leptospirosis and listeriosis has been excluded by means of such immunochemical reactions as the direct and indirect immunofluorescent tests, the solid-phase immunoenzyme assay, the complement fixation test and the agglutination test. The relationship between the cases of hemorrhagic fever in the focus of the outbreak and Crimean-Congo hemorrhagic fever virus has been demonstrated due to the use of the polymerase chain reaction with preliminary reverse transcription.
本文展示了罗斯托夫省出血热病原体的分子生物学检测结果。通过直接和间接免疫荧光试验、固相免疫酶测定、补体结合试验和凝集试验等免疫化学反应，排除了阿斯特拉罕立克次体热、肾综合征出血热、Q热、钩端螺旋体病和李斯特菌病病原体的作用。由于采用了预先逆转录的聚合酶链反应，已证实疫情焦点地区的出血热病例与克里米亚-刚果出血热病毒之间存在关联。
