Göhlmann H W, Weiner J, Schön A, Herrmann R
Zentrum für Molekulare Biologie Heidelberg, Mikrobiologie Universität Heidelberg, Germany.
J Bacteriol. 2000 Jun;182(11):3281-4. doi: 10.1128/JB.182.11.3281-3284.2000.
A highly abundant and heterogeneous small RNA about 205 to 210 bases long named MP200 RNA has been identified in Mycoplasma pneumoniae. It was localized on the genome within a 319-bp-long intergenic space of the pyruvate dehydrogenase (pdh) gene cluster. A database search at the DNA level revealed the highest similarity to a sequence located within the pdh gene cluster of Mycoplasma genitalium that was also shown to be transcribed into two abundant, but smaller RNAs than the ones in Mycoplasma pneumoniae. The RNAs from both M. pneumoniae and M. genitalium have the potential to code for cysteine-rich 29- and 23-amino-acid-long peptides, but so far, these peptides have not been identified experimentally in bacterial protein extracts.
在肺炎支原体中发现了一种高度丰富且异质的小RNA,名为MP200 RNA,其长度约为205至210个碱基。它定位在丙酮酸脱氢酶(pdh)基因簇319 bp长的基因间隔区内的基因组上。在DNA水平上进行的数据库搜索显示,与生殖支原体pdh基因簇内的一个序列相似度最高,该序列也被转录为两种丰富的RNA,但比肺炎支原体中的RNA小。肺炎支原体和生殖支原体的RNA都有可能编码富含半胱氨酸的29个和23个氨基酸长的肽,但到目前为止,这些肽尚未在细菌蛋白提取物中通过实验鉴定出来。