革兰氏阳性细菌枯草芽孢杆菌的全基因组序列。

The complete genome sequence of the gram-positive bacterium Bacillus subtilis.

作者信息

Kunst F, Ogasawara N, Moszer I, Albertini A M, Alloni G, Azevedo V, Bertero M G, Bessières P, Bolotin A, Borchert S, Borriss R, Boursier L, Brans A, Braun M, Brignell S C, Bron S, Brouillet S, Bruschi C V, Caldwell B, Capuano V, Carter N M, Choi S K, Codani J J, Connerton I F, Cummings N J, Daniel R A, Denizot F, Devine K M, Düsterhöft A, Ehrlich S D, Emmerson P T, Entian K D, Errington J, Fabret C, Ferrari E, Foulger D, Fritz C, Fujita M, Fujita Y, Fuma S, Galizzi A, Galleron N, Ghim S Y, Glaser P, Goffeau A, Golightly E J, Grandi G, Guiseppi G, Guy B J, Haga K, Haiech J, Harwood C R, Hènaut A, Hilbert H, Holsappel S, Hosono S, Hullo M F, Itaya M, Jones L, Joris B, Karamata D, Kasahara Y, Klaerr-Blanchard M, Klein C, Kobayashi Y, Koetter P, Koningstein G, Krogh S, Kumano M, Kurita K, Lapidus A, Lardinois S, Lauber J, Lazarevic V, Lee S M, Levine A, Liu H, Masuda S, Mauël C, Médigue C, Medina N, Mellado R P, Mizuno M, Moestl D, Nakai S, Noback M, Noone D, O'Reilly M, Ogawa K, Ogiwara A, Oudega B, Park S H, Parro V, Pohl T M, Portetelle D, Porwollik S, Prescott A M, Presecan E, Pujic P, Purnelle B, Rapoport G, Rey M, Reynolds S, Rieger M, Rivolta C, Rocha E, Roche B, Rose M, Sadaie Y, Sato T, Scanlan E, Schleich S, Schroeter R, Scoffone F, Sekiguchi J, Sekowska A, Seror S J, Serror P, Shin B S, Soldo B, Sorokin A, Tacconi E, Takagi T, Takahashi H, Takemaru K, Takeuchi M, Tamakoshi A, Tanaka T, Terpstra P, Tognoni A, Tosato V, Uchiyama S, Vandenbol M, Vannier F, Vassarotti A, Viari A, Wambutt R, Wedler E, Wedler H, Weitzenegger T, Winters P, Wipat A, Yamamoto H, Yamane K, Yasumoto K, Yata K, Yoshida K, Yoshikawa H F, Zumstein E, Yoshikawa H, Danchin A

出版信息

Nature. 1997 Nov 20;390(6657):249-56. doi: 10.1038/36786.

DOI:10.1038/36786

PMID:9384377

Abstract

Bacillus subtilis is the best-characterized member of the Gram-positive bacteria. Its genome of 4,214,810 base pairs comprises 4,100 protein-coding genes. Of these protein-coding genes, 53% are represented once, while a quarter of the genome corresponds to several gene families that have been greatly expanded by gene duplication, the largest family containing 77 putative ATP-binding transport proteins. In addition, a large proportion of the genetic capacity is devoted to the utilization of a variety of carbon sources, including many plant-derived molecules. The identification of five signal peptidase genes, as well as several genes for components of the secretion apparatus, is important given the capacity of Bacillus strains to secrete large amounts of industrially important enzymes. Many of the genes are involved in the synthesis of secondary metabolites, including antibiotics, that are more typically associated with Streptomyces species. The genome contains at least ten prophages or remnants of prophages, indicating that bacteriophage infection has played an important evolutionary role in horizontal gene transfer, in particular in the propagation of bacterial pathogenesis.

摘要

枯草芽孢杆菌是革兰氏阳性菌中特征最为明确的成员。其4214810个碱基对的基因组包含4100个蛋白质编码基因。在这些蛋白质编码基因中，53%仅有一个拷贝，而基因组的四分之一对应于几个通过基因复制而大幅扩增的基因家族，其中最大的家族包含77个假定的ATP结合转运蛋白。此外，很大一部分遗传能力用于利用多种碳源，包括许多植物衍生分子。鉴于芽孢杆菌菌株能够分泌大量具有工业重要性的酶，五个信号肽酶基因以及分泌装置的几个组成部分的基因的鉴定具有重要意义。许多基因参与次生代谢产物的合成，包括抗生素，而这些次生代谢产物通常与链霉菌属物种相关。该基因组包含至少十个原噬菌体或原噬菌体残余物，这表明噬菌体感染在水平基因转移中发挥了重要的进化作用，特别是在细菌致病机制的传播中。