普氏立克次体向由大肠杆菌ereB基因编码的红霉素抗性的转化。
Transformation of Rickettsia prowazekii to erythromycin resistance encoded by the Escherichia coli ereB gene.
作者信息
Rachek L I, Hines A, Tucker A M, Winkler H H, Wood D O
机构信息
Department of Microbiology and Immunology, Laboratory of Molecular Biology, University of South Alabama College of Medicine, Mobile, Alabama 36688, USA.
出版信息
J Bacteriol. 2000 Jun;182(11):3289-91. doi: 10.1128/JB.182.11.3289-3291.2000.
Rickettsia prowazekii, the etiologic agent of epidemic typhus, is an obligate, intracytoplasmic, parasitic bacterium. Recently, the transformation of this bacterium via electroporation has been reported. However, in these studies identification of transformants was dependent upon either selection of an R. prowazekii rpoB chromosomal mutation imparting rifampin resistance or expression of the green fluorescent protein and flow cytometric analysis. In this paper we describe the expression in R. prowazekii of the Escherichia coli ereB gene. This gene codes for an erythromycin esterase that cleaves erythromycin. To the best of our knowledge, this is the first report of the expression of a nonrickettsial, antibiotic-selectable gene in R. prowazekii. The availability of a positive selection for rickettsial transformants is an important step in the characterization of genetic analysis systems in the rickettsiae.
普氏立克次体是流行性斑疹伤寒的病原体，是一种专性、胞内寄生细菌。最近，已有关于通过电穿孔转化这种细菌的报道。然而，在这些研究中，转化体的鉴定依赖于选择赋予利福平抗性的普氏立克次体rpoB染色体突变，或者依赖于绿色荧光蛋白的表达和流式细胞术分析。在本文中，我们描述了大肠杆菌ereB基因在普氏立克次体中的表达。该基因编码一种可切割红霉素的红霉素酯酶。据我们所知，这是首次报道在普氏立克次体中表达非立克次体的、抗生素可选择的基因。立克次体转化体阳性选择的可用性是立克次体遗传分析系统表征中的重要一步。
Zotero 插件