球形红假单胞菌中5-氨基乙酰丙酸合成酶活性的调控：三硫化物的作用
Control of 5-aminolaevulinate synthetase activity in Rhodopseudomonas spheroides a role for trisulphides.
作者信息
Sandy J D, Davies R C, Neuberger A
出版信息
Biochem J. 1975 Aug;150(2):245-57. doi: 10.1042/bj1500245.
Abstract
- The aminolaevulinate synthetase activator of fresh extracts of semi-anaerobically grown Rhodopseudomonas spheroids was resolved into two fractions by ion-exchange chromatography. One fraction was identified as cystine trisulphide (CySSSCy). Analysis of the other fraction indicated the presence of about equal amounts of glutathione trisulphide (GSSSG) and the mixed trisulphide of glutathione and cystine (GSSSCy). 2. Four further fractions with activator activity were observed on ion-exchange chromatography of extracts prepared by methods similar to those described earlier [Neuberger et al. (1973)Biochem. J. 136,491-499]. These activators were generated by the extraction procedure. Two of them have been identified as trisulphanedisulphonate (S5O62-) and additional cystine trisulphide. 3. For the series of polysulphanedisulphonates (-O3S-Sn-SO3-, n greater than or equal to 1), activator activity at muM concentrations was exhibited only by compounds with n greater than 3. This, together with the results described above, indicates that for a compound R-Sn-R' (where R and R' are organic or inorganic groups) the only structural requirement for activity is n greater than or equal to 3. 4. Oxygenation of a semipanaerobic culture of R. spheroids for 1.5h before harvesting the cells produced a decrease of more than 90% in the cellular content of cystine trisulphide and glutathione trisulphides. 5. Chromatography on DEAE-Sephadex confirmed the presence of multiple forms of aminolaevulinate synthetase in extracts of R. spheroides [Tuboi et al. (1970) Arch.Biochem. Biophys. 138,147-154]. Oxygenation of a semi-anaerobic culture resulted in the disappearance of high-activity enzyme (a-form) and the accumulation of low-activity enzyme (b-form) in the cell. Spontaneous activation [Marriott et al. (1969) Biochem. J. 111,385-394] And activation by cystine trisulphide both resulted in the almost complete conversion of the b-form into the a-form.
摘要
- 通过离子交换色谱法，将半厌氧培养的球形红假单胞菌新鲜提取物中的氨基乙酰丙酸合成酶激活剂分离成两个组分。其中一个组分被鉴定为三硫化胱氨酸（CySSSCy）。对另一个组分的分析表明，存在等量的三硫化谷胱甘肽（GSSSG）以及谷胱甘肽和胱氨酸的混合三硫化物（GSSSCy）。2. 在采用与之前所述方法类似的方法制备的提取物的离子交换色谱分析中，观察到另外四个具有激活剂活性的组分[纽伯格等人（1973年）《生物化学杂志》136卷，491 - 499页]。这些激活剂是由提取过程产生的。其中两个已被鉴定为三硫代二磺酸盐（S5O62-）和额外的三硫化胱氨酸。3. 对于一系列多硫代二磺酸盐（-O3S-Sn-SO3-，n大于或等于1），仅n大于3的化合物在微摩尔浓度下表现出激活剂活性。这与上述结果一起表明，对于化合物R-Sn-R'（其中R和R'为有机或无机基团），活性的唯一结构要求是n大于或等于3。4. 在收获细胞前，将球形红假单胞菌的半厌氧培养物进行1.5小时的氧化处理，导致细胞中三硫化胱氨酸和三硫化谷胱甘肽的含量减少超过90%。5. 在DEAE - 葡聚糖上的色谱分析证实了球形红假单胞菌提取物中存在多种形式的氨基乙酰丙酸合成酶[图博伊等人（1970年）《生物化学与生物物理学报》138卷，147 - 154页]。半厌氧培养物的氧化导致细胞中高活性酶（a型）消失，低活性酶（b型）积累。自发激活[马里奥特等人（1969年）《生物化学杂志》111卷，385 - 394页]以及三硫化胱氨酸激活均导致b型几乎完全转化为a型。
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