增殖细胞核抗原在涎腺黏液表皮样癌中的表达
Proliferating cell nuclear antigen expression in mucoepidermoid carcinoma of salivary glands.
作者信息
Cardoso W P, Denardin O V, Rapoport A, Araújo V C, Carvalho M B
机构信息
Department of Head and Neck Surgery, Heliópolis Hospital, São Paulo, Brazil.
出版信息
Sao Paulo Med J. 2000 May 4;118(3):69-74. doi: 10.1590/s1516-31802000000300004.
CONTEXT
Among the cytological and morphological properties of mucoepidermoid carcinoma, one of the most important criteria for measuring its biological behavior and aggressiveness is cell proliferation. In this way, immunohistochemical markers of cell proliferation have been found to be useful in tumor classification and have formed part of the prognostic and therapeutic studies of these pathologies.
OBJECTIVE
To analyze 11 cases of mucoepidermoid carcinoma (MEC) using the proliferation activity marker (PCNA) and to determine its relationship to the grade of malignancy of these tumors.
DESIGN
Correlation study.
SETTING
Head and Neck Surgery Service of Heliópolis Hospital, São Paulo, Brazil.
SAMPLE
Slides of 11 cases of primary mucoepidermoid carcinomas of salivary glands were prepared according to routine techniques employed in the Oral Pathology Department of the Dentistry Faculty of São Paulo University, Brazil. They were fixed in a 10% formaldehyde solution and stained with hematoxylin and eosin. After this preparation the tumors were classified as low, intermediate and high grade of malignancy, according to the criteria established by Seifert & Sobin and Auclair, Goode & Ellis. The slides were sent for immunohistochemical processing to evaluate the positivity of proliferating cell nuclear antigen using the streptavidin biotin technique.
MAIN MEASUREMENT
The correlation between proliferating cell nuclear antigen expression and the histological malignancy grade in mucoepidermoid carcinoma of salivary glands.
RESULTS
there were 4 cases (36%) of low grade, 4 cases (36%) of intermediate grade and 3 cases (27%) of high grade of malignancy. After a comparative study between histological features and immunohistochemical analysis, significant differences were observed (P < 0.01) for low, intermediate and high grades: 16.04%, 26.98% and 56.98% of proliferating cell nuclear antigen expression in mucoepidermoid carcinoma, respectively.
CONCLUSION
The proliferating cell nuclear antigen expression increases with the grade of malignancy in mucoepidermoid carcinoma of salivary glands.
背景
在黏液表皮样癌的细胞学和形态学特性中，衡量其生物学行为和侵袭性的最重要标准之一是细胞增殖。通过这种方式，已发现细胞增殖的免疫组化标志物在肿瘤分类中有用，并已成为这些病理学预后和治疗研究的一部分。
目的
使用增殖活性标志物（PCNA）分析11例黏液表皮样癌（MEC），并确定其与这些肿瘤恶性程度的关系。
设计
相关性研究。
地点
巴西圣保罗赫利奥波利斯医院头颈外科。
样本
根据巴西圣保罗大学牙科学院口腔病理科采用的常规技术，制备11例唾液腺原发性黏液表皮样癌的切片。将它们固定在10%甲醛溶液中，并用苏木精和伊红染色。制备完成后，根据Seifert & Sobin以及Auclair、Goode & Ellis制定的标准，将肿瘤分为低、中、高恶性等级。将切片送去进行免疫组化处理，使用链霉亲和素生物素技术评估增殖细胞核抗原的阳性情况。
主要测量指标
唾液腺黏液表皮样癌中增殖细胞核抗原表达与组织学恶性等级之间的相关性。
结果
低恶性等级4例（36%），中恶性等级4例（36%），高恶性等级3例（27%）。在组织学特征与免疫组化分析的对比研究后，低、中、高等级之间观察到显著差异（P < 0.01）：黏液表皮样癌中增殖细胞核抗原表达分别为16.04%、26.98%和56.98%。
结论
唾液腺黏液表皮样癌中增殖细胞核抗原的表达随恶性等级增加而升高。
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