Pearlmutter A F, Rapino E, Saffran M
Endocrinology. 1975 Nov;97(5):1336-9. doi: 10.1210/endo-97-5-1336.
Gel filtration of an extract of rat median eminence tissue in 0.1 N HCl on Sephadex G-25 separated two peaks, both with feeble ACTH-releasing activity. Full activity of the extract was regained when both peaks were recombined. This observation suggests that the ACTH-releasing hormone of the hypothalamus requires a co-factor for activity.
用0.1N盐酸提取大鼠正中隆起组织，提取物经葡聚糖凝胶G - 25凝胶过滤后分离出两个峰，二者均具有微弱的促肾上腺皮质激素释放活性。将两个峰重新合并后，提取物恢复了全部活性。这一观察结果表明，下丘脑促肾上腺皮质激素释放激素的活性需要一种辅助因子。
