Chun Y H, Kil J I, Suh Y S, Kim S H, Kim H, Park S H
Institute of Human Genetics, Department of Anatomy, Korea University College of Medicine, Seoul, South Korea.
Cancer Genet Cytogenet. 2000 May;119(1):18-25. doi: 10.1016/s0165-4608(99)00217-4.
Using chromosome painting, a study of chromosomal abnormalities was performed in six gastric carcinoma cell lines (SNU-484, 601, 620, 638, 668, 719) from Korean patients. Each carcinoma cell line had unique modal karyotypic characteristics and showed a variable number of numerical and structural clonal cytogenetic aberrations. SNU-484, SNU-620, and SNU-668 had near-triploidy; SNU-601, SNU-638, and SNU-719 had near-diploidy. The origins of the marker chromosomes of these cell lines were identified by fluorescence in situ hybridization with constructed painting probes. In all of six cell lines, rearrangement of chromosome 17 resulting in partial deletion of 17p (and/or partial duplication of 17q) was found. The most frequent marker was a partial gain of chromosome 7 with the breakpoints on 7q22 and 7q31. The nonrandom rearrangements of chromosomes were also determined on 1q32, 5q11-q22, 8q, 14q22, 14q34, and 15q15; suggesting that they may be the candidate regions for the isolation of the genes related to gastric cancer.
利用染色体涂染技术，对来自韩国患者的6种胃癌细胞系（SNU - 484、601、620、638、668、719）进行了染色体异常研究。每个癌细胞系都有独特的核型特征模式，并显示出数量和结构上不同的克隆性细胞遗传学畸变。SNU - 484、SNU - 620和SNU - 668为近三倍体；SNU - 601、SNU - 638和SNU - 719为近二倍体。通过与构建的涂染探针进行荧光原位杂交，确定了这些细胞系中标记染色体的来源。在所有6个细胞系中，均发现17号染色体重排导致17p部分缺失（和/或17q部分重复）。最常见的标记是7号染色体部分增加，断点位于7q22和7q31。还确定了1q32、5q11 - q22、8q、14q22、14q34和15q15上染色体的非随机重排；这表明它们可能是分离与胃癌相关基因的候选区域。
