酶免疫测定中的一种新方法：用于酶偶联的半抗原马来酰亚胺衍生物。
A novel method in enzyme immunoassay: maleimide derivative of hapten for enzyme coupling.
作者信息
Castro A, Monji N
出版信息
Experientia. 1979 Apr 15;35(4):568-9. doi: 10.1007/BF01922777.
PMID:108135
Abstract
Meta-maleimidobenzoyl derivative of L-thyroxine methyl ester (MBTM) was synthesized and coupled to beta-galactosidase at molar ratio of over 5 to 1. More than 97% of the enzyme was found to be labeled with MBTM. A thyroxine enzyme immunoassay was carried out with sensitivity in 0-10 microgram/100 ml range.
摘要
L-甲状腺素甲酯的间马来酰亚胺苯甲酰衍生物（MBTM）被合成出来，并以超过5比1的摩尔比与β-半乳糖苷酶偶联。发现超过97%的酶被MBTM标记。进行了甲状腺素酶免疫测定，其灵敏度在0 - 10微克/100毫升范围内。
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