• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酶免疫测定中的一种新方法：用于酶偶联的半抗原马来酰亚胺衍生物。

A novel method in enzyme immunoassay: maleimide derivative of hapten for enzyme coupling.

作者信息

Castro A, Monji N

出版信息

Experientia. 1979 Apr 15;35(4):568-9. doi: 10.1007/BF01922777.

DOI:10.1007/BF01922777
PMID:108135
Abstract

Meta-maleimidobenzoyl derivative of L-thyroxine methyl ester (MBTM) was synthesized and coupled to beta-galactosidase at molar ratio of over 5 to 1. More than 97% of the enzyme was found to be labeled with MBTM. A thyroxine enzyme immunoassay was carried out with sensitivity in 0-10 microgram/100 ml range.

摘要

L-甲状腺素甲酯的间马来酰亚胺苯甲酰衍生物（MBTM）被合成出来，并以超过5比1的摩尔比与β-半乳糖苷酶偶联。发现超过97%的酶被MBTM标记。进行了甲状腺素酶免疫测定，其灵敏度在0 - 10微克/100毫升范围内。

相似文献

1
A novel method in enzyme immunoassay: maleimide derivative of hapten for enzyme coupling.酶免疫测定中的一种新方法：用于酶偶联的半抗原马来酰亚胺衍生物。
Experientia. 1979 Apr 15;35(4):568-9. doi: 10.1007/BF01922777.
2
Maleimide derivative of hapten for coupling to enzyme: a new method in enzyme immunoassay.用于与酶偶联的半抗原马来酰亚胺衍生物：酶免疫测定中的一种新方法。
Biochem Biophys Res Commun. 1978 Nov 29;85(2):671-7. doi: 10.1016/0006-291x(78)91214-7.
3
Quantification of digoxin by enzyme immunoassay: synthesis of a maleimide derivative of digoxigenin succinate for enzyme coupling.
Experientia. 1980 Oct 15;36(10):1141-3. doi: 10.1007/BF01976088.
4
Practical enzyme immunoassay for plasma cortisol using beta-galactosidase as enzyme label.使用β-半乳糖苷酶作为酶标记物的血浆皮质醇实用酶免疫测定法。
J Clin Endocrinol Metab. 1980 Feb;50(2):355-9. doi: 10.1210/jcem-50-2-355.
5
The use of N-[beta-(4-diazophenyl)ethyl]maleimide as a heterobifunctional agent in developing enzyme immunoassay for neurotensin.N-[β-(4-重氮苯基)乙基]马来酰亚胺作为一种异双功能试剂在开发神经降压素酶免疫测定中的应用。
Anal Biochem. 1987 Feb 15;161(1):157-63. doi: 10.1016/0003-2697(87)90666-x.
6
The use of a monosaccharide linkage group in a heterologous solid-phase enzyme immunoassay for phenytoin.单糖连接基团在苯妥英异源固相酶免疫测定中的应用。
J Immunol Methods. 1985 Jun 25;80(2):239-53. doi: 10.1016/0022-1759(85)90025-0.
7
Novel preparation method of immunogen for hydrophobic hapten, enzyme immunoassay for daunomycin and adriamycin.
J Immunol Methods. 1981;45(2):195-203. doi: 10.1016/0022-1759(81)90213-1.
8
Synthesis of maleimide derivative of cortisol for enzyme coupling is cortisol enzyme immunoassay.用于酶偶联的皮质醇马来酰亚胺衍生物的合成是皮质醇酶免疫测定法。
Experientia. 1980 Mar 15;36(3):347-9. doi: 10.1007/BF01952319.
9
A heterologous enzyme immunoassay of prostaglandin E2 using a stable enzyme-labeled hapten mimic.
Anal Biochem. 1988 Feb 1;168(2):284-91. doi: 10.1016/0003-2697(88)90320-x.
10
The use of N-[beta-(4-diazophenyl)ethyl]maleimide as a coupling agent in the preparation of enzyme-antibody conjugates.
J Immunol Methods. 1988 May 25;110(1):47-53. doi: 10.1016/0022-1759(88)90081-6.

引用本文的文献

1
Synthesis of maleimide derivative of cortisol for enzyme coupling is cortisol enzyme immunoassay.用于酶偶联的皮质醇马来酰亚胺衍生物的合成是皮质醇酶免疫测定法。
Experientia. 1980 Mar 15;36(3):347-9. doi: 10.1007/BF01952319.

本文引用的文献

1
Determination of sulfhydryl groups with 2,2'- or 4,4'-dithiodipyridine.用2,2'-或4,4'-二硫代二吡啶测定巯基
Arch Biochem Biophys. 1967 Mar;119(1):41-9. doi: 10.1016/0003-9861(67)90426-2.
2
Radioimmunoassay for triiodothyronine (T 3 ): I. Affinity and specificity of the antibody for T 3 .
J Clin Endocrinol Metab. 1971 Sep;33(3):509-16. doi: 10.1210/jcem-33-3-509.
3
Enzyme immunoassay of progesterone at the picogram level using beta-galactosidase as label.以β-半乳糖苷酶为标记物的皮克级孕酮酶免疫测定法。
Biochim Biophys Acta. 1975 Sep 22;403(1):131-8. doi: 10.1016/0005-2744(75)90016-9.
4
Enzyme-linked immunoassay. Conjugation of rabbit anti-(human immunoglobulin G) antibody with beta-D-galactosidase from Escherichia coli and its use for human immunoglobulin G assay.
Eur J Biochem. 1976 Feb 16;62(2):285-92. doi: 10.1111/j.1432-1033.1976.tb10159.x.
5
Enzyme coupled immunoassay of insulin using a novel coupling reagent.使用新型偶联试剂的胰岛素酶联免疫分析
J Biochem. 1976 Jan;79(1):233-6. doi: 10.1093/oxfordjournals.jbchem.a131053.
6
An immuno-enzymatic assay of cortisol using E. coli beta-galactosidase as label.
J Immunol Methods. 1976 Mar;10(2-3):161-70. doi: 10.1016/0022-1759(76)90167-8.
7
Enzyme-linked immunoassay: conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from E. coli and its use for immunoassay.酶联免疫测定：兔免疫球蛋白G的Fab'片段与大肠杆菌β-D-半乳糖苷酶的偶联及其在免疫测定中的应用。
J Immunol. 1976 Jun;116(6):1554-60.