Hinds J A, Pincombe C F, Smith S, Duffy P
J Immunol Methods. 1985 Jun 25;80(2):239-53. doi: 10.1016/0022-1759(85)90025-0.
The preparation is described of monosaccharide-hapten derivatives containing a galactosyl linkage group which bears little structural resemblance to the straight-chain hydrocarbons commonly used for attaching haptens to proteins for use as immunogens. These derivatives are readily synthesised and are coupled to enzymes under mild conditions to produce bridge-heterologous enzyme immunoassays. The use of beta-galactosidase-monosaccharide-phenytoin derivatives in the development of sensitive phenytoin enzyme immunoassays is described and the assays are compared with hapten-heterologous, site-heterologous and homologous phenytoin enzyme immunoassays prepared using other phenytoin derivatives. This technique has application in the development of immunoassays for haptens which have only one functional group to which linkage groups can be attached for covalent coupling of the hapten to proteins.
本文描述了含有半乳糖基连接基团的单糖-半抗原衍生物的制备方法，该连接基团与常用于将半抗原连接到蛋白质上以用作免疫原的直链烃在结构上几乎没有相似之处。这些衍生物易于合成，并在温和条件下与酶偶联以产生桥联异源酶免疫测定。文中描述了β-半乳糖苷酶-单糖-苯妥英衍生物在灵敏的苯妥英酶免疫测定开发中的应用，并将这些测定与使用其他苯妥英衍生物制备的半抗原异源、位点异源和同源苯妥英酶免疫测定进行了比较。该技术可应用于开发针对只有一个可连接连接基团以实现半抗原与蛋白质共价偶联的官能团的半抗原的免疫测定。
