加利福尼亚黑尾鹿腺病毒出血性疾病的血清学检测

Serologic detection of adenoviral hemorrhagic disease in black-tailed deer in California.

作者信息

Lapointe J M, Woods L W, Lehmkuhl H D, Keel M K, Rossitto P V, Swift P K, MacLachlan N J

机构信息

Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California at Davis, 95616, USA.

出版信息

J Wildl Dis. 2000 Apr;36(2):374-7. doi: 10.7589/0090-3558-36.2.374.

DOI:10.7589/0090-3558-36.2.374

PMID:10813622

Abstract

An enzyme-linked immunosorbent assay (ELISA) and a serum neutralization (SN) test were developed to measure serum antibodies against the adenovirus causing hemorrhagic disease in free-ranging and captive experimentally-infected black-tailed deer (Odocoilenus hemionus columbianus) in California (USA). There was a strong (rho = 0.874) and significant (P < 0.0001) correlation between ELISA and SN titers, although the SN assay was more sensitive than the ELISA.

摘要

开发了一种酶联免疫吸附测定（ELISA）和血清中和（SN）试验，用于检测美国加利福尼亚州自由放养和人工感染实验的圈养黑尾鹿（Odocoilenus hemionus columbianus）血清中针对引起出血性疾病的腺病毒的抗体。ELISA和SN滴度之间存在强相关性（rho = 0.874）且具有统计学意义（P < 0.0001），尽管SN试验比ELISA更敏感。