Vaudry H, Chartrel N, Desrues L, Galas L, Kikuyama S, Mor A, Nicolas P, Tonon M C
European Institute for Peptide Research (IFRMP n(o) 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, Mont-Saint-Aignan, France.
Ann N Y Acad Sci. 1999 Oct 20;885:41-56. doi: 10.1111/j.1749-6632.1999.tb08664.x.
In amphibians, alpha-MSH secreted by the pars intermedia of the pituitary plays a pivotal role in the process of skin color adaptation. Reciprocally, the skin of amphibians contains a number of regulatory peptides, some of which have been found to regulate the activity of pituitary melanotrope cells. In particular, the skin of certain species of amphibians harbours considerable amounts of thyrotropin-releasing hormone, a highly potent stimulator of alpha-MSH release. Recently, we have isolated and sequenced from the skin of the frog Phyllomedusa bicolor--a novel peptide named skin peptide tyrosine tyrosine (SPYY), which exhibits 94% similarity with PYY from the frog Rana ridibunda. For concentrations ranging from 5 x 10(-10) to 10(-7) M, SPYY induces a dose-related inhibition of alpha-MSH secretion. At a dose of 10(-7) M, SPYY totally abolished alpha-MSH release. These data strongly suggest the existence of a regulatory loop between the pars intermedia of the pituitary and the skin in amphibians.
在两栖动物中，垂体中间部分分泌的α-促黑素在皮肤颜色适应过程中起关键作用。相反，两栖动物的皮肤含有多种调节肽，其中一些已被发现可调节垂体促黑素细胞的活性。特别是，某些两栖动物物种的皮肤含有大量促甲状腺激素释放激素，这是一种α-促黑素释放的高效刺激剂。最近，我们从双色叶泡蛙的皮肤中分离并测序了一种名为皮肤肽酪氨酸酪氨酸（SPYY）的新型肽，它与泽蛙的PYY有94%的相似性。对于5×10⁻¹⁰至10⁻⁷ M的浓度范围，SPYY诱导α-促黑素分泌呈剂量相关的抑制。在10⁻⁷ M的剂量下，SPYY完全消除了α-促黑素的释放。这些数据有力地表明两栖动物垂体中间部分与皮肤之间存在调节回路。
