利用变性梯度凝胶电泳（DGGE）监测原位生物刺激处理对地下水细菌群落的影响。
Monitoring impact of in situ biostimulation treatment on groundwater bacterial community by DGGE.
作者信息
Iwamoto T, Tani K, Nakamura K, Suzuki Y, Kitagawa M, Eguchi M, Nasu M
机构信息
Environmental Science and Microbiology, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6, Yamada-oka, Suita, Osaka, Japan
出版信息
FEMS Microbiol Ecol. 2000 Apr 1;32(2):129-141. doi: 10.1111/j.1574-6941.2000.tb00707.x.
Changes in bacterial diversity during the field experiment on biostimulation were monitored by denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rDNA fragments. The results revealed that the bacterial community was disturbed after the start of treatment, continued to change for 45 days or 60 days and then formed a relatively stable community different from the original community structure. DGGE analysis of soluble methane monooxygenase (sMMO) hydroxylase gene fragments, mmoX, was performed to monitor the shifts in the numerically dominant sMMO-containing methanotrophs during the field experiment. Sequence analysis on the mmoX gene fragments from the DGGE bands implied that the biostimulation treatment caused a shift of potential dominant sMMO-containing methanotrophs from type I methanotrophs to type II methanotrophs.
通过对聚合酶链反应（PCR）扩增的16S核糖体DNA（rDNA）片段进行变性梯度凝胶电泳（DGGE）分析，监测了生物刺激田间试验期间细菌多样性的变化。结果表明，处理开始后细菌群落受到干扰，持续变化45天或60天，然后形成一个与原始群落结构不同的相对稳定的群落。对可溶性甲烷单加氧酶（sMMO）羟化酶基因片段mmoX进行DGGE分析，以监测田间试验期间数量上占优势的含sMMO甲烷氧化菌的变化。对DGGE条带中的mmoX基因片段进行序列分析表明，生物刺激处理导致潜在的优势含sMMO甲烷氧化菌从I型甲烷氧化菌转变为II型甲烷氧化菌。
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