Elharrar M, Bingen A, Drillien R, Gendrault J L, Steffan A M, Kirn A
Arzneimittelforschung. 1975 Oct;25(10):1586-91.
The i.p. or i.v. injection of frog virus 3 (FV3) in mice produces a hepatitis which leads to the death of the animals within 24 h. This hepatitis is of a purely toxic nature since the virus does not develop at 37 degrees C. The toxic effect of the virus, which can be differentiated from the infectious effect, involves one or more structural proteins. The first pathological changes occur during the first few hours after the injection in the vicinity of the nuclei of the liver parenchyma cells in the form of changes in the chromatin and nucleoplasm. The inhibition of the synthesis of cellular macromolecules and of the function of nuclear enzymes points to the fact that it is the nucleus that is first and foremost attacked. Necrosis and biochemical disturbances in the vicinity of the cytoplasm appear later on. Premedication of the mice with a water-soluble silymarin salt leads to a distinct rise in the survival rate of the animals. The protective function of silymarin is dependent on the dose and on the duration of the premedication. The LD50 of FV3 in those mice which had previously been given silymarin, is approximately three times that of the animals which received no premedication.
给小鼠腹腔注射或静脉注射蛙病毒3（FV3）会引发肝炎，导致动物在24小时内死亡。这种肝炎纯粹是毒性所致，因为该病毒在37摄氏度下不会繁殖。病毒的毒性作用可与感染性作用区分开来，涉及一种或多种结构蛋白。最初的病理变化发生在注射后的最初几个小时内，在肝实质细胞核附近，表现为染色质和核质的变化。细胞大分子合成和核酶功能的抑制表明，首先受到攻击的是细胞核。随后在细胞质附近出现坏死和生化紊乱。用一种水溶性水飞蓟宾盐对小鼠进行预处理，会使动物的存活率显著提高。水飞蓟宾的保护作用取决于剂量和预处理的持续时间。在先前给予水飞蓟宾的小鼠中，FV3的半数致死剂量（LD50）大约是未接受预处理动物的三倍。
