Gendrault J L, Steffan A M, Elharrar M, Kirn A
Arzneimittelforschung. 1979;29(5):786-91.
Pretreatment of mice with silymarin dihemisuccinate sodium salt (SHS-Na) 300 mg/kg 24 and 16 h before infection completely protected against the lethal effect of Frog Virus 3 (FV3). After the inoculation of 1 LD100 of virus it could be shown that: a) the same amount of radioactively labelled virus was found in the liver of control and SHS-Na treated animals, b) in SHS-Na pretreated animals the shut-off of macromolecular syntheses in the liver was considerably less extensive, c) the histological changes in the hepatocyte nuclei were attenuated in SHS-Na pretreated animals and the serum titer of the transaminases did no increase, d) in SHS-Na pretreated mice there were only limited functional alterations of the sinusoidal cells as studied by carbon phagocytosis and the serum titer of the lysosomal enzymes was only slightly modified, e) inhibition of protein synthesis during the period of pretreatment with SHS-Na did not decrease the level of protection. These results allow us to conclude that SHS-Na exerts its protective effect on the liver and that sinusoidal cells are most likely involved in this protection.
在感染前24小时和16小时，用300毫克/千克的水飞蓟宾二琥珀酸钠盐（SHS-Na）对小鼠进行预处理，可完全保护其免受蛙病毒3（FV3）的致死作用。接种1个致死剂量（LD100）的病毒后，结果显示：a）在对照组和SHS-Na处理组动物的肝脏中发现的放射性标记病毒量相同；b）在SHS-Na预处理的动物中，肝脏中大分子合成的阻断程度要小得多；c）在SHS-Na预处理的动物中，肝细胞核的组织学变化减弱，转氨酶的血清滴度没有升高；d）在用碳吞噬法研究时，SHS-Na预处理的小鼠中窦状细胞的功能改变有限，溶酶体酶的血清滴度仅略有改变；e）在SHS-Na预处理期间蛋白质合成的抑制并未降低保护水平。这些结果使我们得出结论，SHS-Na对肝脏发挥保护作用，并且窦状细胞很可能参与了这种保护作用。
