从人胰液中鉴定和纯化一种激肽释放酶，并与其他激肽释放酶进行免疫学比较。

Characterization and purification of a kallikrein from human pancreatic juice and immunological comparison with other kallikreins.

作者信息

Amouric M, Figarella C

出版信息

Hoppe Seylers Z Physiol Chem. 1979 Mar;360(3):457-65. doi: 10.1515/bchm2.1979.360.1.457.

DOI:10.1515/bchm2.1979.360.1.457

Abstract

A prekallikrein has been demonstrated in human pancreatic juice and the active enzyme has been purified from this material. The purification procedure included filtration on Sephadex G-100, chromatography on DEAE-cellulose and affinity chromatography on trypsin-inhibitor Sepharose. The purified kallikrein appeared to be homogeneous by polyacrylamide gel electrophoresis at pH 8.3 and by immunoelectrophoresis. Human pancreatic kallikrein is immunologically different from human plasma kallikrein and from pancreatic kallikreins of other species (hog, cat, rat and dog). Human pancreatic kallikrein has common antigenic determinants with human urinary and submandibular kallikreins but probably not with parotid kallikrein.

摘要

已在人胰液中证实存在前激肽释放酶，并已从该物质中纯化出活性酶。纯化步骤包括在葡聚糖凝胶G - 100上过滤、在二乙氨基乙基纤维素上进行色谱分析以及在胰蛋白酶抑制剂琼脂糖凝胶上进行亲和色谱分析。通过pH 8.3的聚丙烯酰胺凝胶电泳和免疫电泳，纯化的激肽释放酶似乎是均一的。人胰腺激肽释放酶在免疫上与人血浆激肽释放酶以及其他物种（猪、猫、大鼠和狗）的胰腺激肽释放酶不同。人胰腺激肽释放酶与人尿和颌下腺激肽释放酶有共同的抗原决定簇，但可能与腮腺激肽释放酶没有。