Heber H, Geiger R, Heimburger N
Hoppe Seylers Z Physiol Chem. 1978 Jun;359(6):659-69. doi: 10.1515/bchm.1978.359.1.659.
Human plasma kallikrein was isolated from a plasma fraction related to Cohn fraction IV4 by affinity- and Sephadex G-150 chromotography yielding a material with 17.3 TAME-U/A280 unit. The preparation was characterized by immunological and enzymatic methods. Complex formation with alpha2-macroglobulin, C1-inactivator and aprotinin was demonstrated by immunoelectrophoresis. The bradykinin release from high-molecular weight kininogen and its inhibition by antibodies to kallikrein, AT III and AT III-heparin complex were measured using a biological test system (rat uterus). Time dependent inactivation of kallikrein by AT III, and AT III-heparin complex was shown by means of a synthetic kallikrein substrate: Bz-Pro-Phe-Arg-pNan. The same substrate was used to measure the activation of prekallikrein in plasma by kaolin and F XII a. Antibodies raised against kallikrein were shown to inhibit the reaction specifically. A quantitative determination of plasma prekallikrein by electroimmunodiffusion according to Laurell was developed: the plasma concentration in normal individuals was found to be 1.8 - 2.2 TAME-U/ml related to kallikrein activity; this corresponds approximately to 9 - 11 mg antigen/100 ml plasma.
人血浆激肽释放酶是从与Cohn组分IV4相关的血浆级分中，通过亲和色谱和Sephadex G - 150色谱分离得到的，得到一种具有17.3 TAME - U/A280单位的物质。该制剂通过免疫学和酶学方法进行了表征。通过免疫电泳证明了与α2 - 巨球蛋白、C1 - 灭活剂和抑肽酶的复合物形成。使用生物测试系统（大鼠子宫）测量了高分子量激肽原释放缓激肽以及激肽释放酶、抗凝血酶III和抗凝血酶III - 肝素复合物抗体对其的抑制作用。通过合成激肽释放酶底物：Bz - Pro - Phe - Arg - pNan，显示了抗凝血酶III和抗凝血酶III - 肝素复合物对激肽释放酶的时间依赖性灭活作用。相同的底物用于测量高岭土和F XII a对血浆中前激肽释放酶的激活作用。已证明针对激肽释放酶产生的抗体可特异性抑制该反应。开发了一种根据Laurell法通过电免疫扩散对血浆前激肽释放酶进行定量测定的方法：发现正常个体的血浆浓度与激肽释放酶活性相关为1.8 - 2.2 TAME - U/ml；这大约相当于9 - 11 mg抗原/100 ml血浆。
