Shu C H, Tu T Y
Department of Otolaryngology, Taipei Veterans General Hospital, Taiwan, ROC.
Zhonghua Yi Xue Za Zhi (Taipei). 2000 Apr;63(4):288-93.
Latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is a potentially oncogenic protein, able to inhibit differentiation and induce transformation of epithelial cells. The LMP1 gene sequence of EBV in nasopharyngeal carcinoma (NPC) differs from that of EBV in B95-8 cells. A change of nucleotide at the XhoI site results in loss of the restriction site in the EBV LMP1 gene from NPC, and this of EBV is designated as the Taiwan variant. In this study, we further investigated the Taiwan variant of EBV in nasopharyngeal biopsies and throat washings of NPC patients and normal controls.
DNA was extracted from nasopharyngeal biopsies of 22 NPC patients and 40 normal controls, and from throat washings of 28 NPC patients and 78 normal controls. The DNA was amplified by polymerase chain reaction (PCR) using LMP1 gene-specific primers, then the EBV variant was identified by analysis with the XhoI restriction enzyme.
The LMP1 gene was detected in all NPC tumors, in only 25 of 40 normal nasopharyngeal biopsies, and in 12 of 28 and 44 of 78, respectively, throat washings of NPC patients and normal controls. The Taiwan variant was detected in 84.1% to 100% of EBV isolates. The distribution of Taiwan variant EBV was not statistically different between the NPC group and normal controls in either nasopharyngeal biopsies or throat washings.
The results indicate that, based on XhoI restriction site analysis, the Taiwan variant of EBV is the predominant EBV strain in NPC patients and normal individuals in Taiwan.
爱泼斯坦-巴尔病毒（EBV）的潜伏膜蛋白1（LMP1）是一种潜在的致癌蛋白，能够抑制上皮细胞分化并诱导其转化。鼻咽癌（NPC）中EBV的LMP1基因序列与B95-8细胞中EBV的LMP1基因序列不同。XhoI位点的核苷酸变化导致鼻咽癌中EBV LMP1基因的限制性酶切位点丢失，这种EBV被称为台湾变异株。在本研究中，我们进一步调查了台湾NPC患者和正常对照者鼻咽活检组织及咽喉冲洗液中的EBV台湾变异株。
从22例NPC患者和40例正常对照者的鼻咽活检组织，以及28例NPC患者和78例正常对照者的咽喉冲洗液中提取DNA。使用LMP1基因特异性引物通过聚合酶链反应（PCR）扩增DNA，然后用XhoI限制性内切酶分析鉴定EBV变异株。
在所有NPC肿瘤中均检测到LMP1基因，在40例正常鼻咽活检组织中仅25例检测到，在NPC患者和正常对照者的咽喉冲洗液中分别为28例中的12例和78例中的44例检测到。在84.1%至100%的EBV分离株中检测到台湾变异株。在鼻咽活检组织或咽喉冲洗液中，NPC组和正常对照组之间台湾变异株EBV的分布无统计学差异。
结果表明，基于XhoI限制性酶切位点分析，EBV台湾变异株是台湾NPC患者和正常个体中主要的EBV毒株。
