Ballingall K T, Mwangi D M, MacHugh N D, Taracha E L, Totte P, McKeever1 D J
International Livestock Research Institute, P.O. Box 30709, Nairobi, Kenya.
J Immunol Methods. 2000 May 26;239(1-2):85-93. doi: 10.1016/s0022-1759(00)00187-3.
We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFNgamma and activation of immune CD4(+) and CD8(+) T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4(+) T cell clones.
我们描述了一种用于T细胞活化的高灵敏度非放射性检测方法，该方法基于组成型阴性牛内皮细胞在源自活化牛T细胞的上清液存在下培养时，II类主要组织相容性复合体（MHC）表达的快速诱导。我们证明了该检测方法在检测重组牛干扰素γ（rBoIFNγ）以及免疫CD4(+)和CD8(+) T细胞系和克隆分别对特异性抗原和转染的COS-7细胞的活化反应中的有效性。我们还证明了其在鉴定激活免疫CD4(+) T细胞克隆的纯化病原体组分方面的实用性。
