Toye P, Wijngaard P, MacHugh N, Clevers H
International Laboratory for Research on Animal Diseases, Nairobi, Kenya.
J Immunol Methods. 1995 Nov 16;187(1):95-101. doi: 10.1016/0022-1759(95)00173-8.
The studies reported here describe methodology permitting the direct identification of antigens recognized by cytotoxic T lymphocytes. We demonstrated that bovine alloreactive CTL can detect a bovine MHC molecule transiently expressed in a COS cell population in a standard microcytotoxicity assay. We then showed that alloreactive CTL can detect cells expressing the bovine class I MHC molecule in a population of cells transfected with the plasmid containing the corresponding gene plus 100-fold as many plasmids containing an irrelevant gene. In addition, the transiently transfected COS cells can specifically restimulate CTL as detected by a standard microcytotoxicity assay using the target cell line. Overall, the results suggest that COS cells could be employed for the direct screening of an antigen or antigen gene library by immune CTL.
此处报道的研究描述了一种方法，该方法能够直接鉴定细胞毒性T淋巴细胞所识别的抗原。我们证明，在标准的微量细胞毒性试验中，牛同种异体反应性CTL能够检测到在COS细胞群体中瞬时表达的牛MHC分子。随后我们表明，在转染了含有相应基因的质粒以及100倍数量含有无关基因的质粒的细胞群体中，同种异体反应性CTL能够检测到表达牛I类MHC分子的细胞。此外，如通过使用靶细胞系的标准微量细胞毒性试验所检测到的，瞬时转染的COS细胞能够特异性地再次刺激CTL。总体而言，结果表明COS细胞可用于通过免疫CTL直接筛选抗原或抗原基因文库。
