Kerckhaert J A, Hofhuis F M
Ann Immunol (Paris). 1975 Jun-Jul;126C(4):371-82.
The common cell type involved in the humoral and cellular responses is the T-cell. Functionally the T-cell, active in the humoral response, is known as Thelper (Th), the T-cell active in the cellular response as Tcellular (Tc). The present experiments are used to study the relationship between Tc and Th. IP immunization, with suboptimal doses of antigen, did not result in antibody formation but induced both a DH (equals Tc) and a Th population. Th and Tc activity changed in a similar way with increasing doses of antigen. Spleen cells taken from mice, which were immunized under conditions which only invoke cellular activity, were able to function even in small numbers as Th in transfer experiments. Educated thymus cells and cells obtained from cortisone or CY treated donor mice had an increased Th and Tc activity when transferred into irradiated recipients. Education in the presence of a high antigen dose reduced both activities below control levels. Cross reactivity between HRBC and SRBC in the hormoral response is known to be at the Th level. It was demonstrated that this cross reactivity also exists at the Tc level. Induction of DH with one antigen acted as pre-immunization, for the humoral response, on the cross reactive antigen too. These results suggest a correlation between the T-cells involved in DH and the Th for antibody formation.
参与体液免疫和细胞免疫反应的常见细胞类型是T细胞。在功能上，参与体液免疫反应的T细胞被称为辅助性T细胞（Th），参与细胞免疫反应的T细胞被称为细胞毒性T细胞（Tc）。本实验旨在研究Tc和Th之间的关系。用次优剂量抗原进行腹腔内免疫，未导致抗体形成，但诱导了迟发型超敏反应（等同于Tc）和Th群体。随着抗原剂量增加，Th和Tc活性以相似方式变化。从小鼠获取的脾细胞，在仅引发细胞活性的条件下进行免疫，在转移实验中即使数量很少也能发挥Th的功能。受过训练的胸腺细胞以及从经可的松或环磷酰胺处理的供体小鼠获得的细胞，转移至受照射受体后，Th和Tc活性增强。在高剂量抗原存在下进行训练会使两种活性降至对照水平以下。已知在体液免疫反应中，人红细胞（HRBC）和绵羊红细胞（SRBC）之间的交叉反应发生在Th水平。结果表明，这种交叉反应在Tc水平也存在。用一种抗原诱导迟发型超敏反应，对交叉反应抗原的体液免疫反应也起到了预免疫作用。这些结果表明，参与迟发型超敏反应的T细胞与参与抗体形成的Th之间存在相关性。
