Sainte-Marie G, Peng F S
Ann Immunol (Paris). 1975 Jun-Jul;126C(4):501-16.
The present study investigated the migration, in the lymph node, of cells entering the organ via the subcapsular sinus. Thymocytes of rats were labelled with 3H-thymidine and injected into the mediastinal cavity of syngeneic recipients. These animals were sacrificed at various intervals after injection. Five minutes after injection, labelled cells were found in the subcapsular sinus, and even in the peripheral cortex, of the draining mediastinal lymph nodes. The overall of the radioautographic observations reveals a migration of cells from the peripheral cortex towards the deep cortex, and into the medullary cords. The observations indicate that such migration can occur in less than 12 hours. Nevertheless, six hours after the injection, some labelled cells had escaped already the draining nodes and migrated into the other lymphoid organs. It is a fact that some of our results suggest that cells may pass directly from the subcapsular into the medullary sinuses without previously migrating into the nodal parenchyma. As to folliculo-nodules and pseudo-follicles, they exhibited relatively few labelled cells. Moreover, some of our findings indicated that cell migration was much slower in the pseudo-follicles than in the extrafollicular zone.
本研究调查了经被膜下窦进入淋巴结的细胞在淋巴结内的迁移情况。用³H-胸腺嘧啶核苷标记大鼠胸腺细胞，并将其注入同基因受体的纵隔腔。在注射后的不同时间间隔处死这些动物。注射后5分钟，在引流纵隔淋巴结的被膜下窦甚至外周皮质中发现了标记细胞。放射自显影观察的总体结果显示细胞从外周皮质向深层皮质迁移，并进入髓索。观察结果表明，这种迁移可在不到12小时内发生。然而，注射后6小时，一些标记细胞已经从引流淋巴结逸出并迁移到其他淋巴器官。事实上，我们的一些结果表明，细胞可能直接从被膜下进入髓窦，而无需先迁移到淋巴结实质。至于滤泡小结和假滤泡，它们显示出相对较少的标记细胞。此外，我们的一些发现表明，假滤泡中的细胞迁移比滤泡外区域慢得多。
