Cooper A, Liang Z, Castellino F J, Rosen E D
Department of Chemistry and Biochemistry and the WM Keck Center for Transgene Research, University of Notre Dame, Indiana 46556-5670, USA.
Thromb Haemost. 2000 May;83(5):732-5.
The gene encoding murine coagulation factor X (fX) was isolated and characterized from a lamdaFIX II library generated from murine genomic DNA. The 20130 bp sequence contains 18049 nucleotides that extend from the initiating methionine to the polyadenylation site. 1056 nucleotides 5' of the start codon were determined and contain putative start sites for the FX mRNA as well as sites for binding of putative transcription factors. The sequence extends 1024 3' of the polyadenylation site. The gene contains 8 exons and 7 introns which were determined by comparing the mouse FX cDNA and gene sequences. The exonic structure of the gene is similar to that of the other mammalian vitamin K-dependent serine proteases of the coagulation system. These include an exon encoding the prepropepetide, the gla-domain, a short helical stack, two exons for the two EGF domains, the activation pepetide, and two exons encoding the serine protease domain. The 5' sequence of the mouse FX gene overlaps with the 3' region of the FVII gene indicating that the murine FVII and FX gene are arranged in a head to tail arrangement as they are in humans.
从小鼠基因组DNA构建的λFIX II文库中分离并鉴定了编码小鼠凝血因子X（fX）的基因。该20130 bp序列包含18049个核苷酸，从起始甲硫氨酸延伸至聚腺苷酸化位点。起始密码子上游1056个核苷酸已确定，包含FX mRNA的假定起始位点以及假定转录因子的结合位点。该序列在聚腺苷酸化位点下游延伸1024个核苷酸。通过比较小鼠FX cDNA和基因序列确定该基因包含8个外显子和7个内含子。该基因的外显子结构与凝血系统中其他哺乳动物维生素K依赖性丝氨酸蛋白酶的外显子结构相似。这些包括一个编码前原肽的外显子、gla结构域、一个短螺旋堆叠、两个用于两个EGF结构域的外显子、激活肽以及两个编码丝氨酸蛋白酶结构域的外显子。小鼠FX基因的5'序列与FVII基因的3'区域重叠，表明小鼠FVII和FX基因与人一样以头对头的方式排列。
