Liang Z, Cooper A, DeFord M E, Carmeliet P, Collen D, Castellino F J, Rosen E D
Department of Chemistry and Biochemistry and the Center for Transgene Research, University of Notre Dame, Indiana 46556, USA.
Thromb Haemost. 1998 Jul;80(1):87-91.
The cDNA encoding murine coagulation factor X (fX) was isolated and reconstructed from a lambdaZap cDNA library generated from murine liver mRNA. The cDNA contains 1486 bases starting at the 5'-translation initiation codon. It includes an open reading frame of 1443 nucleotides, followed by an 18 residue 3' nontranslated sequence downstream of the first stop codon. and a 3' poly(A) tail. The translation product is composed of a 40-amino acid signal/propeptide region followed by a 441-residue mature protein. The latter is highly homologous to that of human and rat fX. All protein domains of human and rat fX are strictly conserved in mouse fX. The cDNA coding for mouse fX has been expressed in human embryonic kidney 293 cells and generates fX activity measured in a clotting assay using human fX-deficient plasma.
编码小鼠凝血因子X（fX）的cDNA是从由小鼠肝脏mRNA构建的λZap cDNA文库中分离并重建的。该cDNA从5'-翻译起始密码子开始包含1486个碱基。它包括一个1443个核苷酸的开放阅读框，接着是第一个终止密码子下游的18个残基的3'非翻译序列，以及一个3'聚腺苷酸尾。翻译产物由一个40个氨基酸的信号/前肽区域和随后的441个残基的成熟蛋白组成。后者与人及大鼠的fX高度同源。人及大鼠fX的所有蛋白结构域在小鼠fX中严格保守。编码小鼠fX的cDNA已在人胚肾293细胞中表达，并在使用人fX缺陷血浆的凝血试验中产生可测量的fX活性。
