Hayashi H, Hosono N, Kondo M, Hiraoka N, Ikeshiro Y, Shibano M, Kusano G, Yamamoto H, Tanaka T, Inoue K
Gifu Pharmaceutical University, Japan.
Biol Pharm Bull. 2000 May;23(5):602-6. doi: 10.1248/bpb.23.602.
The nucleotide sequences of ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit gene (rbcL) of Glycyrrhiza glabra, G. uralensis, G. inflata, G. echinata, G. macedonica and G. pallidiflora have been determined to construct their phylogenetic tree. Based on these sequences, the six Glycyrrhiza species were divided into two groups: three, G. glabra, G. uralensis, and G. inflata, which produce glycyrrhizin as a major saponin, and the others, G. echinata, G. macedonica and G. pallidiflora, which produce macedonoside C as a major saponin. Among the three glycyrrhizin-producing species, only two nucleotide substitutions were observed between the rbcL sequences of G. glabra and G. uralensis, and the sequence of G. uralensis was identical to that of G. inflata, indicating that G. uralensis and G. inflata are closely related. Among the three macedonoside C-producing species, only one nucleotide substitution was observed between those of G. echinata and G. macedonica, indicating that these two species are also closely related.
已测定光果甘草、乌拉尔甘草、胀果甘草、刺果甘草、马其顿甘草和苍白甘草的1,5-二磷酸核酮糖羧化酶/加氧酶大亚基基因（rbcL）的核苷酸序列，以构建它们的系统发育树。基于这些序列，这六个甘草物种被分为两组：一组是光果甘草、乌拉尔甘草和胀果甘草，它们以甘草甜素作为主要皂苷；另一组是刺果甘草、马其顿甘草和苍白甘草，它们以马其顿苷C作为主要皂苷。在三个产生甘草甜素的物种中，光果甘草和乌拉尔甘草的rbcL序列之间仅观察到两个核苷酸替换，并且乌拉尔甘草的序列与胀果甘草的序列相同，这表明乌拉尔甘草和胀果甘草密切相关。在三个产生马其顿苷C的物种中，刺果甘草和马其顿甘草的序列之间仅观察到一个核苷酸替换，这表明这两个物种也密切相关。
