Chan P J
Center for Fertility and In Vitro Fertilization, Department of Gynecology and Obstetrics, Loma Linda University, California, USA.
Mol Reprod Dev. 2000 Jun;56(2 Suppl):316-8. doi: 10.1002/(SICI)1098-2795(200006)56:2+<316::AID-MRD23>3.0.CO;2-V.
There is a paucity of information about sperm-mediated transmission of exogenous DNA to implanting embryos and cells of the reproductive tract. Preliminary experiments established that sperm has the capacity to actively take in exogenous DNA derived from HPV. In addition, blastocysts also take up exogenous HPV DNA, but in contrast to sperm, the process appears passive. DNA-carrying sperm migrating in an artificial glass tube or excised mouse bicornuate uteri transfected the blastocysts at the remote position using a flip-flop mechanism. There were preferential transmission of the types of HPV DNA but this was not attributed to the gene sequence or the size of the DNA fragments. The internalized DNA became undetectable unless continuous sperm bombardment or pricking took place. Mycoplasma vectors offer a novel way to enhance the transfection of blastocyst with exogenous DNA.
关于精子介导外源性DNA传递至着床胚胎及生殖道细胞的信息匮乏。初步实验证实，精子有能力主动摄取源自人乳头瘤病毒（HPV）的外源性DNA。此外，囊胚也会摄取外源性HPV DNA，但与精子不同的是，这一过程似乎是被动的。携带DNA的精子在人工玻璃管或切除的小鼠双角子宫中迁移，通过一种翻转机制转染远处位置的囊胚。HPV DNA类型存在优先传递现象，但这并非归因于基因序列或DNA片段大小。除非持续进行精子轰击或针刺，否则内化的DNA将无法检测到。支原体载体为增强外源性DNA对囊胚的转染提供了一种新方法。
