Laboratory of Medical Genetics, School of Medicine, University of Ioannina and Medical Genetics and Assisted Reproduction Unit, Department of Obstetrics and Gynecology, University Hospital of Ioannina, Ioannina, Greece.
Department of Veterinary Medicine, University of Maryland, College Park, MD, U.S.A.
In Vivo. 2021 Nov-Dec;35(6):3203-3209. doi: 10.21873/invivo.12615.
BACKGROUND/AIM: Sperm cells are competent to integrate exogenous DNA into their genome. We sought to clarify Human Pappiloma Virus (HPV) internalization in spermatozoa and early preimplantation embryos.
Sperm was incubated with plasmid vectors containing the complete genome of human HPV 16 and HPV 18 tagged with the green fluorescent protein (GFP) gene, to investigate HPV 16 and HPV 18 integration in mouse spermatozoa. Oocytes were in vitro fertilized with preincubated spermatozoa to investigate HPV 16 and HPV 18 potential transfer to mouse embryos.
Spermatozoa were able to internalize constructs of cloned high-risk HPV either as integrated or as episomal DNA. Constructs of cloned HPV can also be transferred to mouse embryos, through in vitro fertilization of the oocytes by mouse spermatozoa.
Viral DNA transmission to the early mouse embryo via sperm, highlights the effect of HPV in reproductive cells and preimplantation development.
背景/目的：精子细胞有能力将外源 DNA 整合到其基因组中。我们旨在阐明人乳头瘤病毒（HPV）在精子和早期胚胎中的内化。
将含有标记有绿色荧光蛋白（GFP）基因的完整人 HPV 16 和 HPV 18 基因组的质粒载体与精子孵育，以研究 HPV 16 和 HPV 18 在小鼠精子中的整合。用预先孵育的精子对卵母细胞进行体外受精，以研究 HPV 16 和 HPV 18 向小鼠胚胎转移的潜能。
精子能够内化作为整合或附加体 DNA 的克隆高危 HPV 的构建体。通过用小鼠精子对卵母细胞进行体外受精，克隆 HPV 的构建体也可以转移到小鼠胚胎中。
病毒 DNA 通过精子向早期小鼠胚胎的传递，突出了 HPV 在生殖细胞和胚胎植入发育中的作用。
