Wallrapp C, Hähnel S, Müller-Pillasch F, Burghardt B, Iwamura T, Ruthenbürger M, Lerch M M, Adler G, Gress T M
Department of Internal Medicine I, University of Ulm, Germany.
Cancer Res. 2000 May 15;60(10):2602-6.
We report the characterization of a novel serine protease of the chymotrypsin family, recently isolated by cDNA-representational difference analysis, as a gene overexpressed in pancreatic cancer. The 2.3-kb mRNA of the gene, named TMPRSS3, is strongly expressed in a subset of pancreatic cancer and various other cancer tissues, and its expression correlates with the metastatic potential of the clonal SUIT-2 pancreatic cancer cell lines. The deduced polypeptide sequence consists of 437 amino acids and exhibits all of the structural features characteristic of serine proteases with trypsin-like activity. TMPRSS3 is membrane bound with a NH2-terminal signal-anchor sequence and a glycosylated extracellular region containing the serine protease domain. Thus, TMPRSS3 is a novel membrane-bound serine protease overexpressed in cancer, which may be of importance for processes involved in metastasis formation and tumor invasion.
我们报道了一种新的胰凝乳蛋白酶家族丝氨酸蛋白酶的特性，该蛋白酶最近通过cDNA代表性差异分析分离得到，是一种在胰腺癌中过表达的基因。该基因名为TMPRSS3，其2.3 kb的mRNA在一部分胰腺癌和各种其他癌组织中强烈表达，并且其表达与克隆的SUIT-2胰腺癌细胞系的转移潜能相关。推导的多肽序列由437个氨基酸组成，具有所有具有胰蛋白酶样活性的丝氨酸蛋白酶的结构特征。TMPRSS3通过一个NH2末端信号锚定序列和一个包含丝氨酸蛋白酶结构域的糖基化细胞外区域与膜结合。因此，TMPRSS3是一种在癌症中过表达的新型膜结合丝氨酸蛋白酶，这可能对转移形成和肿瘤侵袭过程具有重要意义。
