Bourre J, Dumont O, Clément M, Dinh L, Droy-Lefaix M, Christen Y
INSERM U 26, Hôpital Fernand Widal, 200 rue du Faubourg Saint-Denis, 75475 Paris Cedex 10, Paris, France.
Neurosci Lett. 2000 Jun 2;286(2):87-90. doi: 10.1016/s0304-3940(00)01095-8.
The effect of vitamin E deficiency on glutathione peroxidase activity (GPX) and on the activity of a selenoenzyme (phospholipid hydroperoxide glutathione peroxidase (PHGPX) was measured in rat brain and liver. In brain, the activity of both enzymes was in the same range in homogenate and in microsomes. In contrast, in liver homogenate, PHGPX activity was approximately 20 times lower than that of GPX. Very interestingly, PHGPX activity was significantly decreased in brain microsomes by vitamin E deficiency, but slightly significantly increased in liver microsomes. In contrast, GPX activity was not affected in brain by vitamin E deficiency, but was significantly lower in liver homogenate and microsomes. Thus, PHGPX activity is partially controlled by vitamin E in membranes, and PHGPX is probably an enzyme different from GPX.
在大鼠的脑和肝脏中测量了维生素E缺乏对谷胱甘肽过氧化物酶活性（GPX）以及一种含硒酶（磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPX））活性的影响。在脑中，这两种酶在匀浆和微粒体中的活性处于相同范围。相比之下，在肝脏匀浆中，PHGPX活性比GPX活性低约20倍。非常有趣的是，维生素E缺乏使脑微粒体中的PHGPX活性显著降低，但使肝脏微粒体中的PHGPX活性略有显著升高。相反，维生素E缺乏对脑中的GPX活性没有影响，但在肝脏匀浆和微粒体中GPX活性显著降低。因此，膜中的PHGPX活性部分受维生素E控制，并且PHGPX可能是一种不同于GPX的酶。
