Ghelis T, Dellis O, Jeannette E, Bardat F, Cornel D, Miginiac E, Rona J P, Sotta B
Physiologie Cellulaire et Moléculaire des Plantes, UMR CNRS 7632, Université Pierre et Marie Curie (Paris VI), 4 place Jussieu, 75252 Paris, Cedex 05, France.
FEBS Lett. 2000 May 26;474(1):43-7. doi: 10.1016/s0014-5793(00)01574-x.
The abscissic acid (ABA) transduction cascade following the plasmalemma perception was analyzed in intact Arabidopsis thaliana suspension cells. In response to impermeant ABA, anion currents were activated and K(+) inward rectifying currents were inhibited. Anion current activation was required for the ABA specific expression of RAB18. By contrast, specific inhibition of K(+) channels by tetraethylammonium or Ba(2+) did not affect RAB18 expression. Thus, outer plasmalemma ABA perception triggered two separated signaling pathways.
在完整的拟南芥悬浮细胞中分析了质膜感知后脱落酸(ABA)的转导级联反应。响应于不可渗透的ABA,阴离子电流被激活,钾离子内向整流电流被抑制。阴离子电流的激活是RAB18基因ABA特异性表达所必需的。相比之下,用四乙铵或钡离子特异性抑制钾离子通道并不影响RAB18基因的表达。因此,质膜外ABA感知触发了两条独立的信号通路。