Delincée H, Radola B J
Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Dec;28(6):565-79. doi: 10.1080/09553007514551421.
Ribonuclease, irradiated with 60Co gamma-rays in dilute aqueous solution or in the dry state, has been investigated with respect to its charge and size properties. Thin-layer isoelectric focusing revealed extensive change in irradiated RNase; new enzymatically-active components, mainly with isoelectric points lower than in unirradiated RNase were observed. Thin-layer gel chromatography indicated the formation of aggregates which are partially active enzymatically. Aggreation depended on enzyme concentration and was less in more dilute solutions-- at equal degrees of inactivation. Structural damage in the so-called 'native' monomers was revealed by thin-layer isoelectric focusing, their charge properties being distinctly modified.
在稀水溶液或干燥状态下用60Coγ射线辐照的核糖核酸酶，已就其电荷和大小特性进行了研究。薄层等电聚焦显示辐照后的核糖核酸酶发生了广泛变化；观察到了新的具有酶活性的组分，主要是等电点低于未辐照核糖核酸酶的组分。薄层凝胶色谱表明形成了部分具有酶活性的聚集体。聚集取决于酶的浓度，在等程度失活时，较稀溶液中的聚集程度较小。薄层等电聚焦揭示了所谓“天然”单体中的结构损伤，其电荷特性发生了明显改变。
