在60Coγ射线辐照的水溶液中，2'-脱氧鸟苷形成2,5-二氨基-4-羟基-6-(2'-脱氧核糖基)-甲酰胺基嘧啶。

Formation of 2,5-diamino-4-hydroxy-6-(2'-deoxyribosyl)-formamidopyrimidine from 2'-deoxyguanosine in aqueous solution irradiated with 60Co gamma-rays.

作者信息

Ali M, Yamamoto O

机构信息

Department of Biology, Faculty of Sciences, Hiroshima University, Japan.

出版信息

J Radiat Res. 1993 Mar;34(1):12-21. doi: 10.1269/jrr.34.12.

DOI:10.1269/jrr.34.12

PMID:8320657

Abstract

2'-Deoxyguanosine in aqueous solution (5 x 10(-4) mol/dm3) was irradiated with 60Co gamma-rays under N2O. Products were separated by gel chromatography (Cellulofine GC-15-m) and high performance liquid chromatography (Inertsil ODS-2). 2-Amino-4-hydroxy-5-formamido-6-(2'-deoxyribosyl)-aminopyrimidine was one of the main radiolytic products. The formation of 2,5-diamino-4- hydroxy-6-(2'-deoxyribosyl)-formamidopyrimidine was detected, this compound being verified by measuring its ultraviolet absorption spectrum and analyzing its trimethylsilylated derivative by gas chromato-mass spectrometry. Its yield in terms of 2-amino-4-hydroxy-5-formamido-6-(2'-deoxyribosyl)- aminopyrimidine was 1:3.

摘要

在N₂O存在下，用⁶⁰Coγ射线辐照水溶液（5×10⁻⁴mol/dm³）中的2'-脱氧鸟苷。产物通过凝胶色谱法（Cellulofine GC - 15 - m）和高效液相色谱法（Inertsil ODS - 2）进行分离。2 - 氨基 - 4 - 羟基 - 5 - 甲酰胺基 - 6 - （2'-脱氧核糖基）氨基嘧啶是主要的辐射分解产物之一。检测到了2,5 - 二氨基 - 4 - 羟基 - 6 - （2'-脱氧核糖基）甲酰胺基嘧啶的形成，通过测量其紫外吸收光谱并通过气相色谱 - 质谱联用分析其三甲基硅烷基化衍生物对该化合物进行了验证。以2 - 氨基 - 4 - 羟基 - 5 - 甲酰胺基 - 6 - （2'-脱氧核糖基）氨基嘧啶计，其产率为1:3。